Carbohydrate structure characterization by tandem ion mobility mass spectrometry (IMMS)2.
Carbohydrate structure characterization by tandem ion mobility mass spectrometry (IMMS)2.
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DOI：
10.1021/ac303273z
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发表时间：
2013-03-05
影响因子：
7.4
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Li, Hongli;Bendiak, Brad;Siems, William F.;Gang, David R.;Hill, Herbert H., Jr.
将高分辨率离子迁移谱仪与Synapt G2高清晰度质谱仪（HDMS）连接，以进行IMMS-IMMS分析。混合仪器包含电喷雾电离源、两个离子门、环境压力线性离子迁移漂移管、四极质量过滤器、行波离子迁移谱仪（TWIMS）和飞行时间质谱仪。双栅极漂移管离子迁移谱仪（DTIMS）可用于获取传统的IMS谱，但也可选择性地将特定迁移率选择的前体离子转移到Synapt G2 HDMS进行质量过滤（四极杆）。然后，可以将迁移率和质量选择的离子引入碰撞池中进行碎片化，然后用行波离子迁移谱仪对碎片离子进行迁移率分离。这些迁移率分离的碎片离子最终使用飞行时间质谱仪进行质量分析。这导致IMMS-IMMS分析，并提供了一种通过DTIMS和TWIMS评估前体离子的异构异质性的方法，以获得迁移率选择和质量过滤的碎裂模式，并额外获得相应产物离子的行波离子迁移率谱。这种新型IMMS 2仪器能够更详细地研究碳水化合物的结构多样性。DTIMS和TWIMS均能实现寡糖异构体的物理分离，其中DTIMS的分辨率（70~80）高于TWIMS（30~40）。迁移率选择MS/MS光谱，得到的产物离子的TWIMS评价表明，碎片离子的异构形式存在相同的m/z值。
A high resolution ion mobility spectrometer was interfaced to a Synapt G2 high definition mass spectrometer (HDMS) to produce IMMS-IMMS analysis. The hybrid instrument contained an electro-spray ionization source, two ion gates, an ambient pressure linear ion mobility drift tube, a quadrupole mass filter, a traveling wave ion mobility spectrometer (TWIMS) and a time of flight mass spectrometer. The dual gate drift tube ion mobility spectrometer (DTIMS) could be used to acquire traditional IMS spectra, but also could selectively transfer specific mobility selected precursor ions to the Synapt G2 HDMS for mass filtration (quadrupole). The mobility and mass selected ions could then be introduced into a collision cell for fragmentation followed by mobility separation of the fragment ions with the traveling wave ion mobility spectrometer. These mobility separated fragment ions are finally mass analyzed using a time-of-flight mass spectrometer. This results in an IMMS-IMMS analysis and provides a method to evaluate the isomeric heterogeneity of precursor ions by both DTIMS and TWIMS, to acquire a mobility-selected and mass-filtered fragmentation pattern and to additionally obtain traveling wave ion mobility spectra of the corresponding product ions. This new IMMS2 instrument enables the structural diversity of carbohydrates to be studied in greater detail. The physical separation of isomeric oligosaccharide mixtures was achieved by both DTIMS and TWIMS, with DTIMS demonstrating higher resolving power (70~80) than TWIMS (30~40). Mobility selected MS/MS spectra were obtained, and TWIMS evaluation of product ions showed that isomeric forms of fragment ions existed for identical m/z values.
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影响因子：
1.8
作者：
Cerda, BA;Wesdemiotis, C
通讯作者：
Wesdemiotis, C
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
影响因子：
7.4
作者：
CARROLL, DI;DZIDIC, I;HORNING, EC
通讯作者：
HORNING, EC
DOI：
10.1007/s12127-008-0006-5
发表时间：
2008-12-01
影响因子：
--
作者：
Dwivedi, Prabha;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
影响因子：
7.4
作者：
GRIFFIN, GW;DZIDIC, I;HORNING, EC
通讯作者：
HORNING, EC