Imaging of human brain tumor tissue by near-infrared laser coherence tomography.
Imaging of human brain tumor tissue by near-infrared laser coherence tomography.
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DOI：
10.1007/s00701-009-0248-y
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发表时间：
2009-05
影响因子：
2.4
通讯作者：
Giese, A.
中科院分区：
文献类型：
--
作者：
Boehringer, H. J.;Lankenau, E.;Stellmacher, F.;Reusche, E.;Huettmann, G.;Giese, A.
术中残留肿瘤的检测仍然是手术治疗胶质瘤的一个重要挑战。光学技术的新发展提供了非侵入性的高分辨率成像，可以很好地融入神经外科手术的工作流程。使用颅内胶质瘤模型，我们最近表明，时域光学相干断层扫描（OCT）允许正常脑，弥漫性浸润的脑组织，和实体瘤的歧视。OCT成像允许采集2D和3D数据阵列，用于肿瘤与脑界面的多平面分析。在这项研究中，我们分析了人类脑肿瘤的活检标本，我们提出了第一个可行性研究的术中OCT和后图像采集处理的非侵入性成像的大脑和脑肿瘤。我们使用Sirius 713断层扫描仪，其超发光二极管发射近红外中心波长为1，310 nm的光，相干长度为15 µm。光通过光学单模光纤到达改进的OCT适配器，该适配器包含工作距离为10 cm的透镜系统和集成的引导激光器。对导航配准的肿瘤活检进行离体成像，并使用神经导航系统通过标记采集配准光学组织分析的术中部位。脑和脑肿瘤组织的光学相干断层扫描非接触式测量产生宽度为4 mm、深度为1.5-2.0 mm的B扫描图像，轴向和横向光学分辨率为15 µm。OCT成像显示肿瘤与正常脑的不同显微结构和特征信号衰减曲线。图像采集后处理和组织与空气界面的自动检测用于重新对齐A扫描，以补偿由脉冲和呼吸诱导的靶体积移动引起的图像失真。重新排列的图像可以监测扫描线内的强度变化，并有助于选择A扫描平均区域和计算特定感兴趣区域的衰减系数。该可行性研究表明，组织显微结构和光衰减特性的OCT分析可区分正常脑、肿瘤浸润脑、实体瘤和坏死区域。OCT适配器的工作距离和A扫描采集速率在概念上允许将OCT施用器集成到手术显微镜的光路中。这将允许对切除平面进行连续分析，提供光学断层扫描，从而向显微镜视图和关于组织的光衰减特性的信息添加第三维。
Intraoperative detection of residual tumor remains an important challenge in surgery to treat gliomas. New developments in optical techniques offer non-invasive high-resolution imaging that may integrate well into the workflow of neurosurgical operations. Using an intracranial glioma model, we have recently shown that time domain optical coherence tomography (OCT) allows discrimination of normal brain, diffusely invaded brain tissue, and solid tumor. OCT imaging allowed acquisition of 2D and 3D data arrays for multiplanar analysis of the tumor to brain interface. In this study we have analyzed biopsy specimens of human brain tumors and we present the first feasibility study of intraoperative OCT and post-image acquisition processing for non-invasive imaging of the brain and brain tumor. We used a Sirius 713 Tomograph with a superluminescence diode emitting light at a near infrared central wavelength of 1,310 nm and a coherence length of 15 µm. The light is passed through an optical mono mode fiber to a modified OCT adapter containing a lens system with a working distance of 10 cm and an integrated pilot laser. Navigation-registered tumor biopsies were imaged ex vivo and the intraoperative site of optical tissue analysis was registered by marker acquisition using a neuronavigation system. Optical coherence tomography non-contact measurements of brain and brain tumor tissue produced B-scan images of 4 mm in width and 1.5–2.0 mm in depth at an axial and lateral optical resolution of 15 µm. OCT imaging demonstrated a different microstructure and characteristic signal attenuation profiles of tumor versus normal brain. Post-image acquisition processing and automated detection of the tissue to air interface was used to realign A-scans to compensate for image distortions caused by pulse- and respiration-induced movements of the target volume. Realigned images allowed monitoring of intensity changes within the scan line and facilitated selection of areas for the averaging of A-scans and the calculation of attenuation coefficients for specific regions of interest. This feasibility study has demonstrated that OCT analysis of the tissue microstructure and light attenuation characteristics discriminate normal brain, areas of tumor infiltrated brain, solid tumor, and necrosis. The working distance of the OCT adapter and the A-scan acquisition rate conceptually allows integration of the OCT applicator into the optical path of the operating microscopes. This would allow a continuous analysis of the resection plain, providing optical tomography, thereby adding a third dimension to the microscopic view and information on the light attenuation characteristics of the tissue.
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影响因子：
3.7
作者：
Boppart, SA
通讯作者：
Boppart, SA
影响因子：
2.4
作者：
Boehringer, H. J.;Boller, D.;Giese, A.
通讯作者：
Giese, A.
影响因子：
3
作者：
Roper, SN;Moores, MD;Reitze, DH
通讯作者：
Reitze, DH
影响因子：
4.1
作者：
Lacroix, M;Abi-Said, D;Sawaya, R
通讯作者：
Sawaya, R
影响因子：
6.6
作者：
Tearney, GJ;Brezinski, ME;Fujimoto, JG
通讯作者：
Fujimoto, JG