VP15R from infectious spleen and kidney necrosis virus is a non-muscle myosin-II-binding protein
VP15R from infectious spleen and kidney necrosis virus is a non-muscle myosin-II-binding protein
复制标题
来自传染性脾肾坏死病毒的 VP15R 是一种非肌肉肌球蛋白-II 结合蛋白
DOI：
10.1007/s00705-010-0815-9
复制
发表时间：
2010
影响因子：
2.7
通讯作者：
Jianguo He
中科院分区：
文献类型：
--
作者：
Xiaopeng Xu;Ting Lin;Lichao Huang;Shaoping Weng;Wei Wei;Zhongsheng Li;Ling Lu;Zhijian Huang;Jianguo He
鉴定了传染性脾肾坏死病毒(ISKNV)第15个开放阅读框编码的VP15R蛋白。VP15R是一种263个残基的蛋白质，在感染后12小时内首次转录。VP15RmRNA从ISKNV基因组坐标12111开始转录，起始密码子上游167kb。在VP15R序列中没有预测信号肽、跨膜片段或核定位信号序列。VP15R的102-202序列与斑马鱼(Danio Rerio)丝蛋白C的1153-1253序列同源性为29%。免疫荧光和VP15R-GFP融合蛋白亚细胞定位分析表明，VP15R定位于细胞质。Pull-down和MALDI-TOF-TOF/MS分析表明，VP15R可以与非肌肉肌球蛋白II(NM II)结合。免疫共沉淀实验证实，VP15R能与鱼、鼠和人的NM II蛋白重链结合。
VP15R protein, encoded by the 15th open reading frame of infectious spleen and kidney necrosis virus (ISKNV), was identified. VP15R is a 263-residue protein that is first transcribed within 12 h post-infection. The VP15R mRNA is transcribed beginning at ISKNV genomic coordinate 12111, extending 167 bp upstream of the initiation codon. No signal peptides, transmembrane fragments, or nuclear localization signal sequences were predicted in the VP15R sequence. The 102–202 sequence of VP15R is homologous to the 1153–1253 sequence of the filamin C protein ofDanio rerio(zebrafish), with an identity of 29%. Immunofluorescence and VP15R-GFP fusion protein subcellular localization assays showed that VP15R is localized in the cytoplasm. Pull-down and MALDI-TOF–TOF/MS assays demonstrated that VP15R can bind to the non-muscle myosin II (NM II) protein. Co-immunoprecipitation assays confirmed that VP15R can bind to the heavy chains of the NM II protein of mandarin fish, mice, and humans.
登录
查看更多内容
影响因子：
3.7
作者：
G. Darai;H. Delius;Jill Clarke;Heiko Apfel;Paul Schnitzler;R. Flügel
通讯作者：
G. Darai;H. Delius;Jill Clarke;Heiko Apfel;Paul Schnitzler;R. Flügel
影响因子：
0.6
作者：
INOUYE, K;YAMANO, K;SORIMACHI, M
通讯作者：
SORIMACHI, M
影响因子：
3.5
作者：
Takayuki Saitoh;S. Takemura;K. Ueda;H. Hosoya;M. Nagayama;H. Haga;K. Kawabata;Akihiko Yamagishi;Masayuki Takahashi
通讯作者：
Takayuki Saitoh;S. Takemura;K. Ueda;H. Hosoya;M. Nagayama;H. Haga;K. Kawabata;Akihiko Yamagishi;Masayuki Takahashi
影响因子：
4.5
作者：
He, JG;Zeng, K;Chan, SM
通讯作者：
Chan, SM
影响因子：
4.6
作者：
C. A. Mühlen;E. Chan;C. Peebles;H. Imai;K. Kiyosawa;E. Tan
通讯作者：
C. A. Mühlen;E. Chan;C. Peebles;H. Imai;K. Kiyosawa;E. Tan