Transcriptomic and Metabolomic Analyses of Palaemon carinicauda Hepatopancreas in Response to Enterocytozoon hepatopenaei (EHP) Infection
Transcriptomic and Metabolomic Analyses of Palaemon carinicauda Hepatopancreas in Response to Enterocytozoon hepatopenaei (EHP) Infection
复制标题
颈尾长虾肝胰腺对肝对虾肠细胞虫 (EHP) 感染反应的转录组学和代谢组学分析
作者：
Hu GW;Wang WL;Xu K;Wang C;Liu DX;Xu J;Yan BL;Ji NJ;Gao H
无肝肠细胞虫（Enterocytozoon hepatopenaei，EHP）是引起对虾肝胰腺微孢子虫病的一种微孢子虫，是对虾生产中的主要病原。在受影响的物种中，脊尾白虾（Palaemon carinicauda）（以前称为脊尾白虾（Exopalaemon carinicauda））在中国具有重要的商业价值，是研究甲壳类动物的潜在研究模式。然而，关于其对EHP感染的反应的信息很少。因此，本研究使用高通量测序和液相色谱-质谱（LC-MS）分析了脊尾对虾肝胰腺的转录组和代谢组，以确定其在感染早期的反应。转录组学分析确定了730个差异表达基因，其中与EHP感染相关的基因在代谢途径以及解毒和抗氧化途径中富集。京都基因百科全书和基因组途径分析进一步表明，EHP感染后，芳香族化合物的降解、AMP激活的蛋白激酶信号通路和C5分支二元酸代谢显著富集。这些结果可以提供有用的见解EHP对虾在感染的早期阶段的影响，并有助于了解感染后虾生长发育迟缓的机制。
Enterocytozoon hepatopenaei (EHP), a microsporidian responsible for hepatopancreatic microsporidiosis, is a major pathogen in commercial shrimp production. Among the affected species, Palaemon carinicauda (formerly Exopalaemon carinicauda) is commercially important in China and represents a potential research model for studying crustaceans. However, little information is available on its response to EHP infection. Hence, this study analyzed the transcriptome and metabolome of P. carinicauda’s hepatopancreas using high-throughput sequencing and liquid chromatograph-mass spectrometry (LC-MS) to determine its response during the early stage of infection. The transcriptomic analyses identified 730 differentially expressed genes, of which those associated with EHP infection were enriched in metabolic pathways as well as detoxification and antioxidant pathways. In addition, 144 differential metabolites were identified using a combination of positive and negative ion modes in LC-MS. The Kyoto encyclopedia of genes and genomes pathway analyses further indicated that the degradation of aromatic compounds, the AMP-activated protein kinase signaling pathway and C5-branched dibasic acid metabolism were significantly enriched after EHP infection. These results could provide useful insights into the effects of EHP on shrimps during the early stages of infection and help to understand the mechanisms underlying the stunted growth of shrimps after infection.
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影响因子：
3.2
作者：
D. Moreira;R. Silvestre;A. Cordeiro-da-Silva;J. Estaquier;M. Foretz;B. Viollet
通讯作者：
D. Moreira;R. Silvestre;A. Cordeiro-da-Silva;J. Estaquier;M. Foretz;B. Viollet
影响因子：
4.1
作者：
Vontas, JG;Small, GJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
3.9
作者：
Brownsey, RW;Boone, AN;Lee, WM
通讯作者：
Lee, WM
DOI：
10.1016/j.bbalip.2008.12.011
发表时间：
2009-02
影响因子：
4.8
作者：
Flowers, Matthew I.;Ntambi, James M.
通讯作者：
Ntambi, James M.
影响因子：
4.5
作者：
Kumar, T. Sathish;Praveena, P. Ezhil;Jithendran, K. P.
通讯作者：
Jithendran, K. P.