Frequent gain and loss of introns in fungal cytochrome b genes.
Frequent gain and loss of introns in fungal cytochrome b genes.
复制标题
DOI：
10.1371/journal.pone.0049096
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Luo CX
中科院分区：
文献类型：
--
作者：
Yin LF;Hu MJ;Wang F;Kuang H;Zhang Y;Schnabel G;Li GQ;Luo CX
在这项研究中，所有可用的细胞色素B（Cyt B）基因从GOBASE数据库编译和Cyt B基因内含子的进化动力学进行了评估。Cyt B基因内含子在真菌界和一些低等植物中普遍存在，但在染色质纲、原生动物纲和动物纲中普遍缺失或罕见。真菌Cyt B内含子在Cyt B基因的35个位置上被发现，内含子的数量在单个位置上从一个代表性的内含子到位置131处的32个不同的内含子不等，显示出广泛和斑片状的分布。在亲缘关系较远的物种中，许多同源内含子出现在同一位置，而在亲缘关系较近的物种中则不存在，这表明Cyt B基因的内含子经常丢失。另一方面，高度相似的内含子序列中观察到一些远亲物种，而不是在密切相关的物种，这表明这些内含子是独立获得的，可能通过横向转移。内含子丢失和获得事件可能是由核和线粒体之间发生的易位介导的。Southern杂交分析证实部分内含子含有重复序列，可能是转座因子。在Botryotinia fuckeliana的内含子增益防止发展的QoI杀菌剂抗性，这表明内含子的损失和增益事件不一定是有益的，他们的宿主生物。
In this study, all available cytochrome b (Cyt b) genes from the GOBASE database were compiled and the evolutionary dynamics of the Cyt b gene introns was assessed. Cyt b gene introns were frequently present in the fungal kingdom and some lower plants, but generally absent or rare in Chromista, Protozoa, and Animalia. Fungal Cyt b introns were found at 35 positions in Cyt b genes and the number of introns varied at individual positions from a single representative to 32 different introns at position 131, showing a wide and patchy distribution. Many homologous introns were present at the same position in distantly related species but absent in closely related species, suggesting that introns of the Cyt b genes were frequently lost. On the other hand, highly similar intron sequences were observed in some distantly related species rather than in closely related species, suggesting that these introns were gained independently, likely through lateral transfers. The intron loss-and-gain events could be mediated by transpositions that might have occurred between nuclear and mitochondria. Southern hybridization analysis confirmed that some introns contained repetitive sequences and might be transposable elements. An intron gain in Botryotinia fuckeliana prevented the development of QoI fungicide resistance, suggesting that intron loss-and-gain events were not necessarily beneficial to their host organisms.
登录
查看更多内容
引起我国桃褐腐病的褐腐病菌
影响因子：
3.7
作者：
Hu MJ;Cox KD;Schnabel G;Luo CX
通讯作者：
Luo CX
影响因子：
14.9
作者：
COLLINS, RA;REYNOLDS, CA;OLIVE, J
通讯作者：
OLIVE, J
影响因子：
14.9
作者：
Dalgaard, JZ;Klar, AJ;Mian, IS
通讯作者：
Mian, IS
影响因子：
14.9
作者：
Gonzalez, P;Barroso, G;Labarère, J
通讯作者：
Labarère, J
影响因子：
4.1
作者：
Grasso, Valeria;Palermo, Simona;Gisi, Ulrich
通讯作者：
Gisi, Ulrich