In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance.
In-situ and Real-Time Monitoring of the Interaction Between Lysins and Staphylococcus aureus Biofilm by Surface Plasmon Resonance.
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通过表面等离子共振原位实时监测溶素和金黄色葡萄球菌生物膜之间的相互作用。
DOI：
10.3389/fmicb.2021.783472
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wei H
中科院分区：
文献类型：
--
作者：
Hong W;Nyaruaba R;Li X;Liu H;Yang H;Wei H
金黄色葡萄球菌可产生包埋在细胞外聚合物基质中的多层生物膜。这种生物膜难以去除，对抗生素不敏感，容易产生耐药菌株，对环境和健康造成巨大问题。噬菌体溶素通常由催化结构域（CD）和细胞壁结合结构域（CBD）组成，是对抗细菌生物被膜的有力武器。然而，溶素和S。金黄色葡萄球菌生物膜仍不完全清楚。在这项研究中，我们监测了三种溶素（ClyF，ClyC，PlySs2）对芯片上培养的S。金黄色葡萄球菌生物膜，实时，基于表面等离子体共振（SPR）。典型的SPR响应曲线表明，溶素与生物膜结合迅速，生物膜破坏开始于较长的时间。通过1：1结合模型分析，ClyF、ClyC和PlySs2的亲和常数（KD）分别为3.18 ± 0.127 μM、1.12 ± 0.026 μM和15.5 ± 0.514 μM。ClyF和PlySs2具有相同的CBD，但对S.金黄色葡萄球菌生物膜表明，不仅CBD，而且CD影响整个溶素的结合活性。SPR平台可以用于提高我们对溶素和细菌生物膜之间复杂相互作用的理解，包括缔合（吸附）和解离（破坏）。
Staphylococcus aureus can produce a multilayered biofilm embedded in extracellular polymeric matrix. This biofilm is difficult to remove, insensitive to antibiotics, easy to develop drug-resistant strains and causes enormous problems to environments and health. Phage lysin which commonly consists of a catalytic domain (CD) and a cell-wall binding domain (CBD) is a powerful weapon against bacterial biofilm. However, the real-time interaction between lysin and S. aureus biofilm is still not fully understood. In this study, we monitored the interactions of three lysins (ClyF, ClyC, PlySs2) against culture-on-chip S. aureus biofilm, in real-time, based on surface plasmon resonance (SPR). A typical SPR response curve showed that the lysins bound to the biofilm rapidly and the biofilm destruction started at a longer time. By using 1:1 binding model analysis, affinity constants (KD) for ClyF, ClyC, and PlySs2 were found to be 3.18 ± 0.127 μM, 1.12 ± 0.026 μM, and 15.5 ± 0.514 μM, respectively. The fact that ClyF and PlySs2 shared the same CBD but showed different affinity to S. aureus biofilm suggested that, not only CBD, but also CD affects the binding activity of the entire lysin. The SPR platform can be applied to improve our understanding on the complex interactions between lysins and bacterial biofilm including association (adsorption) and disassociation (destruction).
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噬菌体衍生的蛋白质破坏金黄色葡萄球菌生物膜的实时评估。
影响因子：
5.2
作者：
Gutiérrez D;Fernández L;Martínez B;Ruas-Madiedo P;García P;Rodríguez A
通讯作者：
Rodríguez A
影响因子：
4.8
作者：
Low, Lieh Yoon;Yang, Chen;Liddington, Robert
通讯作者：
Liddington, Robert
影响因子：
3.7
作者：
Gutiérrez D;Hidalgo-Cantabrana C;Rodríguez A;García P;Ruas-Madiedo P
通讯作者：
Ruas-Madiedo P
新型不锈钢对金黄色葡萄球菌的抗生物膜形成
DOI：
10.1016/j.msec.2015.03.012
发表时间：
2015-06-01
影响因子：
7.9
作者：
Nan, Li;Yang, Ke;Ren, Guogang
通讯作者：
Ren, Guogang
影响因子：
5.6
作者：
Chen M;Yu Q;Sun H
通讯作者：
Sun H