Low frequency magnetic fields enhance antitumor immune response against mouse H22 hepatocellular carcinoma.
Low frequency magnetic fields enhance antitumor immune response against mouse H22 hepatocellular carcinoma.
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低频磁场增强针对小鼠 H22 肝细胞癌的抗肿瘤免疫反应
DOI：
10.1371/journal.pone.0072411
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Nie Y;Chen Y;Mou Y;Weng L;Xu Z;Du Y;Wang W;Hou Y;Wang T
目的许多研究表明磁场（MF）抑制肿瘤生长并影响免疫系统功能。然而，MF对荷瘤小鼠免疫功能的影响机制仍不清楚。方法本研究通过Balb/c小鼠皮下接种肝癌细胞系H22制备荷瘤小鼠。然后将小鼠暴露于低频 MF（0.4 T，7.5 Hz）30 天。测量存活率、肿瘤生长以及先天性和适应性免疫参数。结果 MF治疗可延长荷瘤小鼠的生存时间（n = 28，p<0.05）并抑制肿瘤生长（n = 9，p<0.01）。此外，这种 MF 抑制了肿瘤诱导的细胞因子的产生，包括白介素 6 (IL-6)、粒细胞集落刺激因子 (G-CSF) 和角质细胞衍生趋化因子 (KC)（n = 9–10，p<0.05 或 0.01）。此外，在小鼠模型中，MF暴露与巨噬细胞和树突状细胞的激活、CD4+T和CD8+T淋巴细胞的增强、Th17/Treg的平衡以及Treg细胞的抑制功能降低相关（n = 9-10，p<0.05或0.01）。结论 MF对肿瘤生长的抑制作用与荷瘤小鼠免疫功能的改善有关。
Objective Many studies have shown that magnetic fields (MF) inhibit tumor growth and influence the function of immune system. However, the effect of MF on mechanism of immunological function in tumor-bearing mice is still unclear. Methods In this study, tumor-bearing mice were prepared by subcutaneously inoculating Balb/c mice with hepatocarcinoma cell line H22. The mice were then exposed to a low frequency MF (0.4 T, 7.5 Hz) for 30 days. Survival rate, tumor growth and the innate and adaptive immune parameters were measured. Results MF treatment could prolong survival time (n = 28, p<0.05) and inhibit tumor growth (n = 9, p<0.01) in tumor-bearing mice. Moreover, this MF suppressed tumor-induced production of cytokines including interleukin-6 (IL-6), granulocyte colony- stimulating factor (G-CSF) and keratinocyte-derived chemokine (KC) (n = 9–10, p<0.05 or 0.01). Furthermore, MF exposure was associated with activation of macrophages and dendritic cells, enhanced profiles of CD4+ T and CD8+ T lymphocytes, the balance of Th17/Treg and reduced inhibitory function of Treg cells (n = 9–10, p<0.05 or 0.01) in the mice model. Conclusion The inhibitory effect of MF on tumor growth was related to the improvement of immune function in the tumor-bearing mice.
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影响因子：
4.7
作者：
Saito T;Nitta H;Kubo O;Yamamoto S;Yamaguchi N;Akiba S;Honda Y;Hagihara J;Isaka K;Ojima T;Nakamura Y;Mizoue T;Ito S;Eboshida A;Yamazaki S;Sokejima S;Kurokawa Y;Kabuto M
通讯作者：
Kabuto M
影响因子：
7.7
作者：
Cakir, Dilek Ulker;Yokus, Beran;Mete, Nuriye
通讯作者：
Mete, Nuriye
影响因子：
1.9
作者：
Chung, Moon-Koo;Yu, Wook-Joon;Myung, Sung-Ho
通讯作者：
Myung, Sung-Ho
影响因子：
4
作者：
Frahm, Jana;Lantow, Magareta;Simko, Myrtill
通讯作者：
Simko, Myrtill
影响因子：
1.7
作者：
Mannerling, Ann-Christine;Simko, Myrtill;Mattsson, Mats-Olof
通讯作者：
Mattsson, Mats-Olof