Engineered NIR-II fluorophores with ultralong-distance molecular packing for high-contrast deep lesion identification.
Engineered NIR-II fluorophores with ultralong-distance molecular packing for high-contrast deep lesion identification.
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DOI：
10.1038/s41467-023-40728-6
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发表时间：
2023-08-18
影响因子：
16.6
通讯作者：
Qian, Jun
中科院分区：
文献类型：
--
作者：
Feng, Zhe;Li, Yuanyuan;Chen, Siyi;Li, Jin;Wu, Tianxiang;Ying, Yanyun;Zheng, Junyan;Zhang, Yuhuang;Zhang, Jianquan;Fan, Xiaoxiao;Yu, Xiaoming;Zhang, Dan;Tang, Ben Zhong;Qian, Jun
长波长近红外-II（NIR-II，900-1880 nm）荧光团的有限信号和由漫射光子引起的强背景使得具有深部组织扰动的体内高对比度荧光成像仍然具有挑战性。在这里，我们开发的NIR-II荧光小分子聚集诱导发射性能，高亮度，最大发射超过1200 nm，通过增强电子的捐赠能力和减少供体-受体（DA）的距离，以补充稀缺的明亮的长波长发射有机染料。D-A-D分子结构的单晶证据表明，当分子堆积距离超过8 μ m时，π-π堆积受到强烈抑制。精心设计的纳米荧光团具有延伸至1900 nm的明亮荧光信号，与背景抑制成像窗口相匹配，使组织图像的信号与背景比达到100以上，组织厚度约为4-6 mm。此外，具有强阴性染色的管腔内病变可以在几乎为零的背景下被识别。该方法可为未来长波长NIR-II分子设计和深部高散射组织的生物医学成像提供新的途径。在深部组织的荧光成像中实现高对比度是具有挑战性的。在这里，作者开发了具有高亮度和延伸至1900 nm的发射的NIR-II荧光小分子，使深部组织的体内成像具有增强的信号与背景比。
The limited signal of long-wavelength near-infrared-II (NIR-II, 900–1880 nm) fluorophores and the strong background caused by the diffused photons make high-contrast fluorescence imaging in vivo with deep tissue disturbed still challenging. Here, we develop NIR-II fluorescent small molecules with aggregation-induced emission properties, high brightness, and maximal emission beyond 1200 nm by enhancing electron-donating ability and reducing the donor-acceptor (D-A) distance, to complement the scarce bright long-wavelength emissive organic dyes. The convincing single-crystal evidence of D-A-D molecular structure reveals the strong inhibition of the π-π stacking with ultralong molecular packing distance exceeding 8 Å. The delicately-designed nanofluorophores with bright fluorescent signals extending to 1900 nm match the background-suppressed imaging window, enabling the signal-to-background ratio of the tissue image to reach over 100 with the tissue thickness of ~4–6 mm. In addition, the intraluminal lesions with strong negatively stained can be identified with almost zero background. This method can provide new avenues for future long-wavelength NIR-II molecular design and biomedical imaging of deep and highly scattering tissues. To achieve high-contrast in fluorescence imaging of deep tissues is challenging. Here, the authors develop NIR-II fluorescent small molecules with high brightness and emission extending to 1900 nm, enabling in vivo imaging of deep tissues with enhanced signal-to-background ratios.
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影响因子：
16.6
作者：
Chen J;Chen L;Wu Y;Fang Y;Zeng F;Wu S;Zhao Y
通讯作者：
Zhao Y
影响因子：
10
作者：
Li, Yi-Xuan;Su, Shih-Po;Chan, Yang-Hsiang
通讯作者：
Chan, Yang-Hsiang
影响因子：
28.1
作者：
Bruns, Oliver T.;Bischof, Thomas S.;Bawendi, Moungi G.
通讯作者：
Bawendi, Moungi G.
与激发激光器相匹配的短波红外多胸荧光团可实时实时非侵入性，多色在体内成像。
影响因子：
21.8
作者：
Cosco ED;Spearman AL;Ramakrishnan S;Lingg JGP;Saccomano M;Pengshung M;Arús BA;Wong KCY;Glasl S;Ntziachristos V;Warmer M;McLaughlin RR;Bruns OT;Sletten EM
通讯作者：
Sletten EM
影响因子：
16.6
作者：
Chen, Muxiong;Feng, Zhe;Fan, Xiaoxiao;Sun, Jun;Geng, Weihang;Wu, Tianxiang;Sheng, Jinghao;Qian, Jun;Xu, Zhengping
通讯作者：
Xu, Zhengping