A H(2)O(2)-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging.
A H(2)O(2)-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging.
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DOI：
10.1038/s41467-021-27233-4
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发表时间：
2021-11-25
影响因子：
16.6
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Chen J;Chen L;Wu Y;Fang Y;Zeng F;Wu S;Zhao Y
开发高质量的 NIR-II 荧光团（发射波长为 1000-1700nm）用于体内成像具有重要意义。苯并噻二唑核心荧光团是一类重要的 NIR-II 染料，但持续存在的局限性（例如水环境中聚集引起的猝灭和不可激活的响应）仍然是其生物学应用的主要障碍。在这里，我们设计了一种可激活的纳米探针来解决这些限制。一种名为 BTPE-NO2 的分子探针是通过将苯并噻二唑核心与两个作为疏水分子转子的四亚苯基连接起来，然后在核心两端掺入两个硝基苯基氧代乙酰胺单元作为识别部分和荧光猝灭剂来合成的。然后采用 FDA 批准的两亲性聚合物 Pluronic F127 封装分子 BTPE-NO2，形成纳米探针 BTPE-NO2@F127。病变部位病理水平的H2O2裂解硝基苯氧乙酰胺基团并激活探针，从而产生强荧光发射（950~1200nm）和超声信号，用于炎症性疾病的多模式成像。因此，纳米探针可以作为一种强大的工具，通过 NIR-II 荧光和多光谱光声断层扫描 (MSOT) 成像来检测和成像疾病部位。此外，可以获得三维 MSOT 图像，用于可视化和定位病灶。第二生物窗口中的荧光成像对于体内应用具有优势。在这里，作者合成了一种分子纳米探针，它在结合 H2O2 时激活，产生强荧光 NIR-II 发射和超声信号，用于炎症性疾病的多模式成像。
Developing high-quality NIR-II fluorophores (emission in 1000–1700 nm) for in vivo imaging is of great significance. Benzothiadiazole-core fluorophores are an important class of NIR-II dyes, yet ongoing limitations such as aggregation-caused quenching in aqueous milieu and non-activatable response are still major obstacles for their biological applications. Here, we devise an activatable nanoprobe to address these limitations. A molecular probe named BTPE-NO2 is synthesized by linking a benzothiadiazole core with two tetraphenylene groups serving as hydrophobic molecular rotors, followed by incorporating two nitrophenyloxoacetamide units at both ends of the core as recognition moieties and fluorescence quenchers. An FDA-approved amphiphilic polymer Pluronic F127 is then employed to encapsulate the molecular BTPE-NO2 to render the nanoprobe BTPE-NO2@F127. The pathological levels of H2O2 in the disease sites cleave the nitrophenyloxoacetamide groups and activate the probe, thereby generating strong fluorescent emission (950~1200 nm) and ultrasound signal for multi-mode imaging of inflammatory diseases. The nanoprobe can therefore function as a robust tool for detecting and imaging the disease sites with NIR-II fluorescent and multispectral optoacoustic tomography (MSOT) imaging. Moreover, the three-dimensional MSOT images can be obtained for visualizing and locating the disease foci. Fluorescent imaging in the second biological window has advantages for in vivo applications. Here, the authors synthesise a molecular nanoprobe which activates upon binding H2O2, generating both strong fluorescent NIR-II emission and ultrasound signal for multi-mode imaging of inflammatory diseases.
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影响因子：
10.3
作者：
Chen, Yen-Ta;Chiang, Hsin-Ju;Yip, Hon-Kan
通讯作者：
Yip, Hon-Kan
影响因子：
16.6
作者：
Jo J;Lee CH;Kopelman R;Wang X
通讯作者：
Wang X
影响因子：
14
作者：
Feng, Guangxue;Tay, Chor Yong;Liu, Bin
通讯作者：
Liu, Bin
影响因子：
16.6
作者：
Antaris AL;Chen H;Diao S;Ma Z;Zhang Z;Zhu S;Wang J;Lozano AX;Fan Q;Chew L;Zhu M;Cheng K;Hong X;Dai H;Cheng Z
通讯作者：
Cheng Z
影响因子：
4.5
作者：
Caro, Pilar;Gomez, Jose;Barja, Gustavo
通讯作者：
Barja, Gustavo