Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics.
Differentiation of human mesenchymal stem cells on niobium-doped fluorapatite glass-ceramics.
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DOI：
10.1016/j.dental.2011.10.010
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发表时间：
2012-03
期刊：
影响因子：
5
通讯作者：
Denry, Isabelle L.
中科院分区：
文献类型：
--
作者：
Kushwaha, Meenakshi;Pan, Xueliang;Holloway, Julie A.;Denry, Isabelle L.
关键词：
我们的目标是表征人间充质干细胞 (hMSC) 对掺铌氟磷灰石基玻璃陶瓷 (FAp) 的反应。该玻璃通过在 1525°C 下两次熔化 3 小时来制备，并浇铸成圆柱形锭，然后切成圆盘并进行热处理以促进氟磷灰石亚微米晶体的结晶。使用组织培养聚苯乙烯（TCP）作为对照。 FAp 圆盘的表面经过热处理、研磨或蚀刻。 3小时时评估初始细胞附着。在第 1 天、第 4 天和第 8 天收集增殖和碱性磷酸酶 (ALP) 表达数据。在第 2 天和第 4 天使用 SEM 检查细胞形态。通过茜素红染色和 SEM 评估矿化。热处理、蚀刻或研磨表面上的初始细胞附着与阳性对照组相似 (p>0.05)。在第 1 天到第 8 天之间，经过热处理、蚀刻或研磨的表面被活细胞覆盖的面积百分比显着增加 (p<0.05)。与 TCP 对照相比，第 8 天时组间的细胞覆盖率没有显着差异。 SEM 显示分布良好的多边形细胞，具有大量丝状伪足，要么附着在陶瓷表面，要么连接到邻近的细胞。与 FAp 和对照的生长培养基相比，成骨培养基中第 8 天的 ALP 表达显着更高。第35天时，通过SEM观察到FAp盘被茜素红染色呈阳性，并且富含钙的矿化颗粒与原纤维相关。hMSC在铌掺杂的FAp玻璃陶瓷上表现出良好的附着、增殖和分化。
Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a niobium-doped fluorapatite-based glass-ceramic (FAp). The glass was prepared by twice melting at 1525°C for 3h, and cast into cylindrical ingots later sectioned into discs and heat-treated to promote crystallization of fluorapatite submicrometer crystals. Tissue culture polystyrene (TCP) was used as control. The surface of the FAp discs was either left as-heat treated, ground or etched. Initial cell attachment was assessed at 3h. Proliferation and alkaline phosphatase (ALP) expression data was collected at days 1, 4, and 8. Cell morphology was examined using SEM, at days 2 and 4. Mineralization was evaluated by Alizarin Red staining and SEM. Initial cell attachment on as heat-treated, etched, or ground surfaces was similar to that of the positive control group (p>0.05). The percentage of area covered by living cells increased significantly on as heat-treated, etched, or ground surfaces between days 1 and 8 (p<0.05). There was no significant difference amongst groups in cell coverage at day 8, compared to TCP control. SEM revealed well spread polygonal cells with numerous filopodia, either attached to the ceramic surface or connected to neighboring cells. ALP expression at day 8 was significantly higher in osteogenic media compared to growth media on both FAp and control. FAp discs stained positively with alizarin red and calcium-rich mineralized granules associated with fibrils were observed by SEM at day 35. hMSCs displayed excellent attachment, proliferation, and differentiation on niobium-doped FAp glass-ceramic.
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影响因子：
9.7
作者：
Bhadang, K. A.;Holding, C. A.;Haynes, D. R.
通讯作者：
Haynes, D. R.
DOI：
10.1002/jbm.b.30295
发表时间：
2005-10-01
影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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