Comparative Study on Measured Variables and Sensitivity to Bone Microstructural Changes Induced by Weightlessness Between In Vivo and Ex Vivo Micro-CT Scans
Comparative Study on Measured Variables and Sensitivity to Bone Microstructural Changes Induced by Weightlessness Between In Vivo and Ex Vivo Micro-CT Scans
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体内和离体显微 CT 扫描测量变量和对失重引起的骨微结构变化的敏感性的比较研究
DOI：
10.1007/s00223-010-9422-8
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发表时间：
2010
影响因子：
4.2
通讯作者：
Fan, Yu Bo
中科院分区：
文献类型：
--
作者：
Li, De Yu;Niu, Hai Jun;Sun, Lian Wen;Wang, Chao;Pu, Fang;Fan, Yu Bo
根据实验设计，微型 CT 可用于检查体内或离体（新鲜切除或福尔马林固定）的骨骼。在这项研究中，我们研究了体内和离体扫描之间通过显微CT测量的变量是否存在差异，以及哪种扫描对模拟失重引起的骨微结构的变化更敏感。使用大鼠尾部悬挂来模拟失重条件。通过体内和离体（新鲜并用福尔马林固定）的微型 CT 扫描来自正常或尾部悬浮大鼠的相同骨骼。然后，分析骨矿物质密度（BMD）和微观结构特征。结果表明，股骨和胫骨的活体、新鲜和福尔马林固定骨扫描中，骨小梁的微观结构参数不存在显着差异，但 BMD 存在差异。另一方面，通过显微CT测量的悬尾大鼠的大部分参数与对照组相比有所恶化。离体扫描似乎对尾部悬吊引起的骨微结构变化比体内扫描更敏感。一般来说，结果表明体内和离体（新鲜和固定）获得的值具有可比性，因此无论扫描类型如何，都可以对不同研究的实验结果进行有意义的比较。此外，这项研究表明，在评估失重状态下的骨微观结构时，最好使用离体扫描。然而，研究人员可以根据实验的目标和要求选择任何类型的扫描。
Depending on the experimental design, micro-CT can be used to examine bones either in vivo or ex vivo (excised fresh or formalin-fixed). In this study we investigated if differences exist in the variables measured by micro-CT between in vivo and ex vivo scans and which kind of scan is more sensitive to the changes of bone microstructure induced by simulated weightlessness. Rat tail suspension was used to simulate the weightless condition. The same bone from either normal or tail-suspended rats was scanned by micro-CT both in vivo and ex vivo (fresh and fixed by formalin). Then, bone mineral density (BMD) and microstructural characteristics were analyzed. The results showed that no significant differences existed in the microstructural parameters of trabecular bone among in vivo, fresh, and formalin-fixed bone scans from both femurs and tibias, although BMD exhibited differences. On the other hand, most parameters of the tail-suspended rats measured by micro-CT deteriorated compared with controls. Ex vivo scanning appeared to be more sensitive to bone microstructural changes induced by tail suspension than in vivo scanning. In general, the results indicate that values obtained in vivo and ex vivo (fresh and fixed) are comparable, thus allowing for meaningful comparison of experimental results from different studies irrespective of the type of scans. In addition, this study suggests that it is better to use ex vivo scanning when evaluating bone microstructure under weightlessness. However, researchers can select any type of scan depending upon the objective and the demands of the experiment.
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DOI：
10.1007/978-3-540-45456-4_24
发表时间：
2007-12
期刊：
--
影响因子：
--
作者：
Yebin Jiang;J. Jacobson;H. Genant;Jenny Zhao
通讯作者：
Yebin Jiang;J. Jacobson;H. Genant;Jenny Zhao
影响因子：
6.2
作者：
J. Kinney;N. Lane;D. Haupt
通讯作者：
J. Kinney;N. Lane;D. Haupt
影响因子：
6.2
作者：
Khosla, S;Riggs, BL;Melton, LJ
通讯作者：
Melton, LJ
影响因子：
3.2
作者：
Laib, A;Barou, O;Rügsegger, P
通讯作者：
Rügsegger, P
影响因子：
4.1
作者：
Waarsing, JH;Day, JS;Weinans, H
通讯作者：
Weinans, H