Unilateral Silver-Loaded Silk Fibroin Difunctional Membranes as Antibacterial Wound Dressings.
Unilateral Silver-Loaded Silk Fibroin Difunctional Membranes as Antibacterial Wound Dressings.
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单侧载银丝素蛋白双功能膜作为抗菌伤口敷料
DOI：
10.1021/acsomega.1c02035
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发表时间：
2021-07-13
期刊：
影响因子：
4.1
通讯作者：
Ge S
中科院分区：
文献类型：
--
作者：
Shao J;Cui Y;Liang Y;Liu H;Ma B;Ge S
丝素蛋白因其良好的生物相容性被广泛应用于创面敷料。为了提高敷料的抗菌性能，经常添加银（Ag）。然而，过量的银可能引起细胞毒性并抑制伤口愈合。因此，本研究旨在通过一种简化的逐层技术，开发一种双层膜，以降低细胞毒性，同时保持银的抗菌性能。膜由富银SF层（Ag-SF）和纯SF层组成。单侧载银膜在银浓度大于0.06 mg/mL时表现出良好的抗菌性能，且两侧的抗菌性能相当。相反，膜的SF侧表现出比Ag-SF侧更低的细胞毒性。进一步对Ag-SF/SF层厚度比的研究表明，当Ag0.12-SF/SF膜的厚度比为1:3时，在SF侧具有较高的细胞相容性，同时具有较强的抗菌性能。此外，Ag0.12-SF/SF1:3膜的SF侧可促进人包皮成纤维细胞中I型胶原和转化生长因子-β mRNA的表达。Ag0.12-SF/SF1:3膜的SF侧在体内显著促进感染创面愈合。因此，单侧负载和简化的逐层制备技术为平衡载银材料的细胞毒性和抗菌性能提供了一种有效的方法，从而为银的应用形成了更广阔的治疗窗口。单侧载银丝素双功能膜作为伤口敷料有进一步临床前探索的潜力。
Silk fibroin (SF) has been widely used as wound dressings due to its good biocompatibility. To enhance the antibacterial properties of the dressings, silver (Ag) is often added. However, an overdose of Ag may cause cytotoxicity and inhibit wound healing. Therefore, this study aimed to develop a two-layered membrane to reduce cytotoxicity while maintaining the antibacterial properties of Ag through a simplified layer-by-layer technique. The membranes comprised an Ag-rich SF layer (Ag-SF) and a pure SF layer. The unilateral Ag-loaded membranes showed efficient antibacterial properties at doses above 0.06 mg/mL Ag, and the antibacterial properties were comparable on both sides. In contrast, the SF sides of the membranes showed lower cytotoxicity than the Ag-SF sides of the membranes. Further studies on the thickness ratio of Ag-SF/SF layers revealed that Ag0.12-SF/SF membranes with a ratio of 1:3 had high cytocompatibility on the SF sides while holding a strong antibacterial property. Besides, the SF sides of the Ag0.12-SF/SF1:3 membranes promoted the expression levels of collagen I and transforming growth factor-β mRNA in human foreskin fibroblasts. The SF sides of the Ag0.12-SF/SF1:3 membranes significantly promoted the healing of infected wounds in vivo. Therefore, unilateral loading with the simplified layer-by-layer preparation technique provided an effective method to balance the cytotoxicity and the antibacterial property of Ag-loaded materials and thus form a broader therapeutic window for Ag applications. The unilateral Ag-loaded silk fibroin difunctional membranes have the potential to be further preclinically explored as wound dressings.
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影响因子：
2.9
作者：
Lichtman, Michael K.;Otero-Vinas, Marta;Falanga, Vincent
通讯作者：
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DOI：
10.1016/j.ijbiomac.2015.12.055
发表时间：
2016-04-01
影响因子：
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作者：
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发表时间：
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影响因子：
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作者：
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DOI：
10.1007/s10856-014-5152-1
发表时间：
2014-05-01
影响因子：
3.7
作者：
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通讯作者：
Lin, Jui-Hsiang
影响因子：
9.7
作者：
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