Role of antibody dependent cell mediated cytotoxicity (ADCC) in Sm-p80-mediated protection against Schistosoma mansoni.
Role of antibody dependent cell mediated cytotoxicity (ADCC) in Sm-p80-mediated protection against Schistosoma mansoni.
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DOI：
10.1016/j.vaccine.2012.09.026
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发表时间：
2012-11-06
期刊：
影响因子：
5.5
通讯作者：
Siddiqui, Afzal A.
中科院分区：
文献类型：
--
作者：
Torben, Workineh;Ahmad, Gul;Zhang, Weidong;Nash, Stewart;Le, Loc;Karmakar, Souvik;Siddiqui, Afzal A.
关键词：
血吸虫病是发展中国家和前往流行国家的国际旅行者的主要健康问题。现有的控制血吸虫病的策略迄今为止取得的成功有限。在现有的控制措施中增加一种有效的疫苗将大大有利于减少这种疾病的影响。在这方面，Sm-p80介导的保护对肠道血吸虫病引起的曼氏血吸虫病已被观察到有希望在两个动物模型的感染和疾病。本研究揭示了抗体依赖性细胞介导的细胞毒性（ADCC）在Sm-p80介导的保护作用中的作用，特别是在消除肺期血吸虫童虫中的作用。这是使用肺灌洗液细胞和肺细胞实现的，所述肺细胞分离自用和不用重组疫苗制剂中配制的Sm-p80免疫的小鼠。使用免疫血清与肺灌洗细胞的细胞毒性测定中观察到显著差异，与对照组相比，其显示出51%以上的童虫杀伤和上清液中一氧化氮水平升高。
Schistosomiasis is a major health problem in the developing world and for international travelers to the endemic countries. Existing strategies to control schistosomiasis have had limited successes so far. The addition of an effective vaccine in existing control measures would be greatly beneficial in reducing the impact of the disease. In this regard, Sm-p80 mediated protection against intestinal schistosomiasis caused by Schistosoma mansoni has been observed to be promising in two animal models of infection and disease. In this study, the role of antibody dependent cell mediated cytotoxcity (ADCC) was deciphered in Sm-p80-mediated protection especially in the elimination of lung stage schistosomula. This was achieved using lung lavage cells and lung cells that were isolated from mice immunized with and without Sm-p80 formulated in a recombinant vaccine formulation. Significant differences were observed in cytotoxicity assays using immune sera with the lung lavage cells which showed 51% more killing of schistosomula and elevated levels of nitric oxide in the supernatants were detected compared to controls.
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影响因子：
5.5
作者：
HotaMitchell, S;Siddiqui, AA;Podesta, RB
通讯作者：
Podesta, RB
影响因子：
1.7
作者：
Hotez, Peter J.;Brown, Ami Shah
通讯作者：
Brown, Ami Shah
影响因子：
1.3
作者：
Keating, John H.;Wilson, R. Alan;Skelly, Patrick J.
通讯作者：
Skelly, Patrick J.
DOI：
10.1016/0035-9203(91)90231-m
发表时间：
1991-07-01
影响因子：
2.2
作者：
KIMANI, G;CHUNGE, CN;MUGAMBI, M
通讯作者：
MUGAMBI, M
影响因子：
4.3
作者：
CHUNG, PR;ASCH, HL;BRUCE, JI
通讯作者：
BRUCE, JI