Differentiation of short, single-stranded DNA homopolymers in solid-state nanopores.
Differentiation of short, single-stranded DNA homopolymers in solid-state nanopores.
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DOI：
10.1021/nn4014388
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发表时间：
2013-05-28
期刊：
影响因子：
17.1
通讯作者：
Drndic, Marija
中科院分区：
文献类型：
--
作者：
Venta, Kimberly;Shemer, Gabriel;Puster, Matthew;Rodriguez-Manzo, Julio A.;Balan, Adrian;Rosenstein, Jacob K.;Shepard, Ken;Drndic, Marija
关键词：
在过去的二十年中，监测单分子通过纳米级孔时离子电流调制的新技术使许多单分子研究成为可能。虽然生物纳米孔最近显示出在单个DNA分子中解析单个核苷酸的能力，但固态纳米孔的类似发展滞后，这是由于在制造与生物纳米孔相似尺寸的稳定纳米孔以及实现足够低噪声和高带宽记录方面的挑战。在这里，我们展示了小的氮化硅纳米孔（直径为0.8至2纳米，在5至8纳米厚的膜上）可以解决短（30碱基）ssDNA均聚聚合物(poly(dA), poly（dC), poly(dT)）产生的离子电流信号之间的差异，当与测量电子相结合时，允许在1 MHz带宽下实现优于10的信噪比。虽然用氮化硅纳米孔识别分子内DNA序列需要进一步提高纳米孔的灵敏度和噪声水平，但均聚物的分化是固态纳米孔发展的一个重要里程碑。
In the last two decades, new techniques that monitor ionic current modulations as single molecules pass through a nanoscale pore have enabled numerous single-molecule studies. While biological nanopores have recently shown the ability to resolve single nucleotides within individual DNA molecules, similar developments with solid-state nanopores have lagged, due to challenges both in fabricating stable nanopores of similar dimensions as biological nanopores and in achieving sufficiently low-noise and high-bandwidth recordings. Here we show that small silicon nitride nanopores (0.8 to 2-nm-diameter in 5 to 8-nm-thick membranes) can resolve differences between ionic current signals produced by short (30 base) ssDNA homopolymers (poly(dA), poly(dC), poly(dT)), when combined with measurement electronics that allow a signal-to-noise ratio of better than 10 to be achieved at 1 MHz bandwidth. While identifying intramolecular DNA sequences with silicon nitride nanopores will require further improvements in nanopore sensitivity and noise levels, homopolymer differentiation represents an important milestone in the development of solid-state nanopores.
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