The nuclear proteome of Trypanosoma brucei.
The nuclear proteome of Trypanosoma brucei.
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DOI：
10.1371/journal.pone.0181884
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kramer S
中科院分区：
文献类型：
--
作者：
Goos C;Dejung M;Janzen CJ;Butter F;Kramer S
布鲁氏锥虫是一种鞭毛虫，由采采蝇传播到哺乳动物的血液中。这种寄生虫通过直接导致非洲昏睡病和通过感染家畜间接对人类健康产生巨大影响。锥虫的生物学涉及一些非常不寻常的、核局部化的过程。这些包括从组蛋白变体定义的区域开始的无经典启动子的多顺反子转录，所有转录本的反式剪接到剪接的前导RNA的外显子，RNA聚合酶I转录一些非常丰富的蛋白质和抗原变异，细胞表面蛋白变体表达的开关，使寄生虫能够抵抗其哺乳动物宿主的免疫系统。在这里，我们提供了布鲁氏原环锥虫的核蛋白质组，这一阶段位于采采蝇中肠内。与全细胞裂解物相比，我们进行了定量无标记质谱分析，获得了764个显着核浓缩蛋白的分数。与几个实验表征的核和非核结构和途径的蛋白质组的比较证实了数据集的高质量：蛋白质组包含大约80%的所有核蛋白和不到2%的假阳性。利用基序富集法，我们发现KRxR的氨基酸序列存在于大量的核蛋白中。KRxR是一个经典的真核单部分核定位信号的亚基，可能负责动质形目物种中蛋白质的核定位。作为原理的证明，我们通过表达EYFP融合蛋白证实了六种以前未知定位的蛋白质的核定位。虽然布鲁氏锥虫几个细胞器的蛋白质组数据已经公布，但我们的核蛋白质组填补了研究锥虫生物学，特别是与核相关的过程的知识的一个重要空白。
Trypanosoma brucei is a protozoan flagellate that is transmitted by tsetse flies into the mammalian bloodstream. The parasite has a huge impact on human health both directly by causing African sleeping sickness and indirectly, by infecting domestic cattle. The biology of trypanosomes involves some highly unusual, nuclear-localised processes. These include polycistronic transcription without classical promoters initiated from regions defined by histone variants, trans-splicing of all transcripts to the exon of a spliced leader RNA, transcription of some very abundant proteins by RNA polymerase I and antigenic variation, a switch in expression of the cell surface protein variants that allows the parasite to resist the immune system of its mammalian host. Here, we provide the nuclear proteome of procyclic Trypanosoma brucei, the stage that resides within the tsetse fly midgut. We have performed quantitative label-free mass spectrometry to score 764 significantly nuclear enriched proteins in comparison to whole cell lysates. A comparison with proteomes of several experimentally characterised nuclear and non-nuclear structures and pathways confirmed the high quality of the dataset: the proteome contains about 80% of all nuclear proteins and less than 2% false positives. Using motif enrichment, we found the amino acid sequence KRxR present in a large number of nuclear proteins. KRxR is a sub-motif of a classical eukaryotic monopartite nuclear localisation signal and could be responsible for nuclear localization of proteins in Kinetoplastida species. As a proof of principle, we have confirmed the nuclear localisation of six proteins with previously unknown localisation by expressing eYFP fusion proteins. While proteome data of several T. brucei organelles have been published, our nuclear proteome closes an important gap in knowledge to study trypanosome biology, in particular nuclear-related processes.
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影响因子：
64.5
作者：
Akiyoshi B;Gull K
通讯作者：
Gull K
DOI：
10.1074/mcp.m900038-mcp200
发表时间：
2009-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
DeGrasse JA;DuBois KN;Devos D;Siegel TN;Sali A;Field MC;Rout MP;Chait BT
通讯作者：
Chait BT
影响因子：
14.9
作者：
Cristodero, Marina;Clayton, Christine E.
通讯作者：
Clayton, Christine E.
影响因子：
14.9
作者：
Gassen A;Brechtefeld D;Schandry N;Arteaga-Salas JM;Israel L;Imhof A;Janzen CJ
通讯作者：
Janzen CJ
影响因子：
3.4
作者：
Colasante, Claudia;Ellis, Margrit;Voncken, Frank
通讯作者：
Voncken, Frank