Differential expression of the chicken Pax‐1 and Pax‐9 Gene: In situ hybridization and immunohistochemical analysis
Differential expression of the chicken Pax‐1 and Pax‐9 Gene: In situ hybridization and immunohistochemical analysis
复制标题
鸡 Pax-1 和 Pax-9 基因的差异表达：原位杂交和免疫组织化学分析
作者：
H. Peters;U. Doll;J. Niessing
我们报告了鸡Pax-1（chPax-1）和Pax-9（chPax-9）基因的克隆，部分序列分析和时空表达，这两个基因是PAX基因家族的两个密切相关的成员。chPax-1基因编码2.0和4.3 kb的RNA和42 kD的蛋白质，而chPax-9的基因产物由1.9和3.1 kb的转录物和39 kD的蛋白质表示。原位杂交和免疫组织化学分析显示chPax-1在发育中的胸带、硬骨节腹侧部分的细胞、脊索管的硬骨节细胞以及发育后期的椎间盘的硬骨节衍生细胞中表达。在寰椎和枢椎周围的间充质中以及未成熟椎体的软骨细胞中检测到的其他chPax-1表达结构域（迄今为止尚未报道小鼠Pax-1）与小鼠Pax-1突变体波状和波状短尾中尚未解释的畸形相关。在胚胎和成人胸腺的上皮细胞以及发育中的椎间盘细胞中检测到chPax-1和chPax-9的重叠表达。然而，与chPax-1不同的是，chPax-9在那些被认为产生椎体的脊索周围硬化体细胞中不表达。此外，chPax-9基因产物在跖骨和内胚层衍生物的间充质的限定区域中检测到，即，在发育中的咽和胚胎及成人食管的衬里上皮中。©1995 Wiley利斯公司
We report the cloning, partial sequence analysis, and spatiotemporal expression of the chicken Pax‐1 (chPax‐1) and Pax‐9 (chPax‐9) gene, two closely related members of the paired box‐containing (PAX) gene family. The chPax‐1 gene encodes RNAs of 2.0 and 4.3 kb and a 42 kD protein while the gene products of chPax‐9 are represented by 1.9 and 3.1 kb transcripts and a 39 kD protein. In situ hybridization and immunohistochemical analyses reveal chPax‐1 expression in the developing pectoral girdle, in cells of the ventral part of sclerotomes, in sclerotome cells of the perichordal tube, and, later in development, in sclerotome‐derived cells of the intervertebral disks. Other chPax‐1 expression domains detected in the mesenchyme surrounding the atlas and axis and in chondrocytes of immature vertebral bodies, so far unreported for mouse Pax‐1, correlate with as yet unexplained malformations in the mouse Pax‐1 mutant undulated and Undulated‐short tail. Overlapping expression of chPax‐1 and chPax‐9 is detected in epithelial cells of the embryonic and adult thymus and in cells of the developing intervertebral disks. Unlike chPax‐1, however, chPax‐9 is not expressed in those perichordal sclerotome cells which are thought to give rise to vertebral bodies. Furthermore, chPax‐9 gene products are detected in circumscribed areas of mesenchyme in the metatarsus and in entodermal derivatives, i.e., in the lining epithelium of the developing pharynx and of the embryonic and adult esophagus. ©1995 Wiley‐Liss, Inc.
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影响因子：
4.6
作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
通讯作者：
Andreas W. Puschel;Peter Gruss;M. Westerfield
DOI：
10.1073/pnas.89.4.1179
发表时间：
1992-02-15
影响因子：
11.1
作者：
DRESSLER, GR;DOUGLASS, EC
通讯作者：
DOUGLASS, EC
影响因子：
4.6
作者：
H. Nornes;G. Dressler;E. Knapik;U. Deutsch;Peter Gruss
通讯作者：
H. Nornes;G. Dressler;E. Knapik;U. Deutsch;Peter Gruss
DOI：
10.1089/dna.1983.2.329
发表时间：
1983-01-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
通讯作者：
BAXTER, JD