Resveratrol metabolites modify adipokine expression and secretion in 3T3-L1 pre-adipocytes and mature adipocytes.
Resveratrol metabolites modify adipokine expression and secretion in 3T3-L1 pre-adipocytes and mature adipocytes.
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DOI：
10.1371/journal.pone.0063918
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Portillo MP
中科院分区：
文献类型：
--
作者：
Eseberri I;Lasa A;Churruca I;Portillo MP
由于白藜芦醇的生物利用度低，确定其代谢产物是否发挥任何有益作用是一个有趣的问题。3T3-L1成熟前脂肪细胞在分化过程中用25µM白藜芦醇或其代谢物处理，3T3-L1成熟脂肪细胞用10µM白藜芦醇或其代谢物处理24小时。Real Time RT-PCR检测脂联素、瘦素、内脏脂肪素和apelin的基因表达，ELISA法测定其在培养液中的浓度。白藜芦醇降低瘦素mRNA水平，提高脂联素mRNA水平。它引起了瘦素分泌的相同变化。反式白藜芦醇-3- o -葡糖苷和反式白藜芦醇-4 ' - o -葡糖苷增加了apelin和visfatin mRNA水平。反式白藜芦醇-3- o -硫酸盐可降低瘦素mRNA水平，提高瘦素和内脏脂肪素mRNA水平。本研究首次发现白藜芦醇代谢物对脂肪因子的表达和分泌具有调节作用。由于白藜芦醇有减少体内脂肪积累和改善胰岛素敏感性的报道，并且考虑到这些作用部分是由所分析的脂肪因子的变化介导的，因此可能提出白藜芦醇代谢物在白藜芦醇的这些有益作用中起作用。
Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue. 3T3-L1 maturing pre-adipocytes were treated during differentiation with 25 µM of resveratrol or with its metabolites and 3T3-L1 mature adipocytes were treated for 24 hours with 10 µM resveratrol or its metabolites. The gene expression of adiponectin, leptin, visfatin and apelin was assessed by Real Time RT-PCR and their concentration in the incubation medium was quantified by ELISA. Resveratrol reduced mRNA levels of leptin and increased those of adiponectin. It induced the same changes in leptin secretion. Trans-resveratrol-3-O-glucuronide and trans-resveratrol-4′-O-glucuronide increased apelin and visfatin mRNA levels. Trans-resveratrol-3-O-sulfate reduced leptin mRNA levels and increased those of apelin and visfatin. The present study shows for the first time that resveratrol metabolites have a regulatory effect on adipokine expression and secretion. Since resveratrol has been reported to reduce body-fat accumulation and to improve insulin sensitivity, and considering that these effects are mediated in part by changes in the analyzed adipokines, it may be proposed that resveratrol metabolites play a part in these beneficial effects of resveratrol.
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影响因子：
3.5
作者：
Baarine, Mauhamad;Thandapilly, Sijo Joseph;Latruffe, Norbert
通讯作者：
Latruffe, Norbert
DOI：
10.1042/bj20091857
发表时间：
2010-07-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Calamini B;Ratia K;Malkowski MG;Cuendet M;Pezzuto JM;Santarsiero BD;Mesecar AD
通讯作者：
Mesecar AD
影响因子：
7
作者：
Griffin, TJ;Gygi, SP;Aebersold, R
通讯作者：
Aebersold, R
DOI：
10.1111/j.1749-6632.2010.05845.x
发表时间：
2011-01-01
期刊：
RESVERATROL AND HEALTH
影响因子：
--
作者：
Baile, Clifton A.;Yang, Jeong-Yeh;Della-Fera, Mary Anne
通讯作者：
Della-Fera, Mary Anne
影响因子：
4.5
作者：
Alberdi G;Rodríguez VM;Miranda J;Macarulla MT;Arias N;Andrés-Lacueva C;Portillo MP
通讯作者：
Portillo MP