Development and In Vivo Characterization of Probiotic Lysate-Treated Chitosan Nanogel as a Novel Biocompatible Formulation for Wound Healing.
Development and In Vivo Characterization of Probiotic Lysate-Treated Chitosan Nanogel as a Novel Biocompatible Formulation for Wound Healing.
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DOI：
10.1155/2020/8868618
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发表时间：
2020
影响因子：
--
通讯作者：
Gholami A
中科院分区：
文献类型：
--
作者：
Ashoori Y;Mohkam M;Heidari R;Abootalebi SN;Mousavi SM;Hashemi SA;Golkar N;Gholami A
伤口愈合是对组织损伤的生理反应，在替换受损组织方面发挥着至关重要的作用。益生菌产生的有价值的化合物具有抗菌和抗炎活性、免疫调节作用以及促进伤口愈合的血管生成特性。壳聚糖纳米结构具有多种特性，使其能够快速生产成凝胶、支架、纳米颗粒、珠子和海绵结构，可纳入伤口愈合过程。在当前的研究中，从相应培养物的培养物中制备了三种纳米凝胶制剂，其由负载益生菌上清液（罗伊氏乳杆菌、发酵乳杆菌和枯草芽孢杆菌纳豆）的壳聚糖纳米凝胶组成。壳聚糖纳米凝胶之前已通过 Zetasizer、FTIR 和 TEM 进行了表征。通过评估 Sprague-Dawley 大鼠的伤口闭合和组织学试验来评估制备的制剂的有效性和敷料活性。结果表明，所有益生菌裂解物制剂在伤口愈合过程中都具有优势。然而，纳豆枯草芽孢杆菌具有更好的伤口愈合质量，这在病理检查中是众所周知的。益生菌裂解物纳米凝胶的良好效果，包括合理的伤口闭合率、良好的伤口外观以及通过体内检查进行的令人满意的组织学观察，表明它是用于伤口愈合目的的有前途的候选者。
Wound healing is a physiological reaction to tissue injuries which plays a crucial role in replacing the destroyed tissues. Probiotics produce valuable compounds that possess antibacterial and anti-inflammatory activities, immunomodulatory effects, and angiogenesis traits leading to the promotion of wound healing. Chitosan nanostructures have versatile properties making them quickly produced into gels, scaffolds, nanoparticles, beads, and sponge structures that can be incorporated into wound healing processes. In the current study, three formulations from nanogel consisting of probiotic supernatant (Lactobacillus reuteri, Lactobacillus fermentum, and Bacillus subtilis sp. natto)-loaded chitosan nanogels were prepared from the culture of corresponding cultures. The chitosan nanogels were previously characterized by Zetasizer, FTIR, and TEM. The prepared formulations' effectiveness and dressing activity were assessed by evaluating wound closure and histological trials in Sprague-Dawley rats. The results indicated that all probiotic lysate formulations have advantages over the wound healing process. However, Bacillus subtilis natto has a better wound healing quality, which is well known in pathology examination. The favorable effects of probiotic lysate nanogels, including the reasonable wound closing rate, good wound appearance, and satisfactory histological observation via in vivo examination, suggest it as a promising nominee for wound healing purposes.
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影响因子：
4.6
作者：
Emadi F;Amini A;Gholami A;Ghasemi Y
通讯作者：
Ghasemi Y
影响因子：
--
作者：
Azarang A;Farshad O;Ommati MM;Jamshidzadeh A;Heydari R;Abootalebi SN;Gholami A
通讯作者：
Gholami A
影响因子：
4.6
作者：
Brandi, Jessica;Cheri, Samuele;Cecconi, Daniela
通讯作者：
Cecconi, Daniela
影响因子：
1.1
作者：
Polak-Berecka, M.;Wasko, A.;Kreft, A.
通讯作者：
Kreft, A.
影响因子：
5.4
作者：
Twetman, S.;Keller, M. K.;Pedersen, A. M. Lynge
通讯作者：
Pedersen, A. M. Lynge