Cholecystokinin Activates C‐Fos Expression in Hypothalamic Oxytocin and Corticotropin‐Releasing Hormone Neurons
Cholecystokinin Activates C‐Fos Expression in Hypothalamic Oxytocin and Corticotropin‐Releasing Hormone Neurons
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胆囊收缩素激活下丘脑催产素和促肾上腺皮质激素释放激素神经元中 C-Fos 的表达
DOI：
10.1111/j.1365-2826.1991.tb00264.x
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发表时间：
1991
影响因子：
3.2
通讯作者：
G. Hoffman
中科院分区：
文献类型：
--
作者：
J. Verbalis;E. Stricker;A. G. Robinson;G. Hoffman
通过分析视上核和室旁核免疫细胞化学特征神经元中c - fos抗原的表达，研究了全身给药CCK对下丘脑催产素、抗利尿激素和促肾上腺皮质激素释放激素神经元的影响。CCK （100μg/kg腹腔注射）使细胞核c‐fos免疫细胞化学染色明显增加，在注射后60 ~ 90 min达到峰值。C‐fos在视上核的大多数大细胞催产素神经元和室旁核的所有大细胞亚区均有表达，但在这两个区域的加压素神经元中均无表达。C‐fos在室旁核的几个旁细胞分支中也有表达：在内侧和外侧的催产素神经元中，但在背侧的旁细胞分支中没有，在内侧旁细胞分支的促肾上腺皮质激素释放激素神经元中也有表达。注射低剂量CCK时，c‐fos的表达与CCK对垂体后叶催产素分泌的模式密切相关，激活阈值为1 μg/kg，接近最大反应阈值为10 μg/kg，最大反应阈值为100 μg/kg。这些研究表明，在全身CCK给药后，下丘脑大细胞神经元中c‐fos的表达模式反映了这些神经元的神经分泌反应，无论是在分泌肽的特异性还是分泌强度方面。他们还证明，全身给药CCK激活了parparocellular催产素和促肾上腺皮质激素释放激素神经元中的c - fos表达，因此可能导致大脑中这些神经元的终端场分泌催产素和促肾上腺皮质激素释放激素。
The effect of systemically‐administered Cholecystokinin octapeptide (CCK) on hypothalamic oxytocin, vasopressin, and corticotropin‐releasing hormone neurons was studied by analysis of c‐fos antigen expression in immunocytochemically‐characterized neurons in the supraoptic and paraventricular nuclei. CCK (100μg/kg intraperitoneally) caused a marked increase in nuclear c‐fos immunocytochemical staining, which peaked at 60 to 90 min after injection. C‐fos expression was found in most magnocellular oxytocin neurons in the supraoptic nucleus and in all magnocellular subdivisions of the paraventricular nucleus, but in no vasopressin neurons in either area. C‐fos expression was also found in several parvocellular subdivisions of the paraventricular nucleus: in oxytocin neurons within the medial and lateral, but not the dorsal, parvocellular subdivisions, and in corticotropin‐releasing hormone neurons in the medial parvocellular subdivision. Injection of lower doses of CCK showed that c‐fos expression closely paralleled the pattern of pituitary oxytocin secretion in response to CCK, with a threshold for activation at 1 μg/kg, near maximal responses by 10 μg/kg, and maximal responses by 100 μg/kg. These studies demonstrate that the pattern of c‐fos expression in hypothalamic magnocellular neurons following systemic CCK administration mirrors the neurosecretory response of these neurons, both with regard to specificity for the peptides secreted as well as intensity of secretion. They also demonstrate that systemic CCK administration activates c‐fos expression in parvocellular oxytocin and corticotropin‐releasing hormone neurons, and therefore likely causes secretion of oxytocin and corticotropin‐releasing hormone within the brain at the terminal fields of these neurons.
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影响因子：
56.9
作者：
SAGAR, SM;SHARP, FR;CURRAN, T
通讯作者：
CURRAN, T
影响因子：
4.1
作者：
SWANSON, LW;SAWCHENKO, PE;VALE, WW
通讯作者：
VALE, WW
辣椒素预处理可减弱大鼠对胆囊收缩素的多种反应。
DOI：
10.1016/0165-1838(88)90101-4
发表时间：
1988
期刊：
Journal of the autonomic nervous system
影响因子：
--
作者：
McCann,MJ;Verbalis,JG;Stricker,EM
通讯作者：
Stricker,EM
影响因子：
56.9
作者：
PEDERSEN, CA;ASCHER, JA;PRANGE, AJ
通讯作者：
PRANGE, AJ
胆囊收缩素和胃扩张激活大鼠下丘脑中的催产素能细胞。
DOI：
10.1152/ajpregu.1987.253.4.r661
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Renaud,LP;Tang,M;McCann,MJ;Stricker,EM;Verbalis,JG
通讯作者：
Verbalis,JG