Anticoagulant effects on the evaluation of CD23 expression in cells by monoclonal antibodies
Anticoagulant effects on the evaluation of CD23 expression in cells by monoclonal antibodies
复制标题
单克隆抗体评价细胞内CD23表达的抗凝作用
DOI：
10.1080/10428194.2022.2038379
复制
发表时间：
2022
影响因子：
2.6
通讯作者：
Yasuda Takeshi
中科院分区：
文献类型：
--
作者：
Tajima Katsushi;Tanaka Kiwa;Takeda Hikaru;Moriya Mika;Ueki Tetsuya;Ogata Shinya;Maeda Kunihiko;Yasuda Takeshi
CD 23是一种II型跨膜糖蛋白，属于C型（Ca 2+依赖性）凝集素样超家族。CD 23蛋白以可溶形式从细胞表面裂解，除了调节IgE应答和B细胞活化和生长外，还发挥多效性活性[1]。CD 23广泛分布于许多正常细胞中，包括T和B淋巴细胞、单核细胞和滤泡树突状细胞，其表达受到各种刺激的不同调节[2，3]。CD 23表达是区分慢性淋巴细胞性白血病/小淋巴细胞性淋巴瘤（CLL/SLL）、套细胞淋巴瘤（MCL）和其他惰性淋巴瘤的有用且相关的诊断标志物，并且与其临床病理学发现和预后相关[4，5]。因此，通过流式细胞术免疫表型分析（FCIP）和免疫组化（IHC）对靶细胞中CD 23表达的精确评价和分析是严格诊断及其相关最适当治疗选择的关键和关键过程[6，7]。CD 23与IgE的相互作用也是预防过敏性疾病的治疗靶向机制[8]。在临床环境中，通过直接免疫荧光染色FCIP分析，在抗凝全血、骨髓抽吸物或相关肌肉组织的单细胞悬液中评价表面CD 23表达[9-12]。因此，抗CD 23的一抗可以直接和同时与靶细胞在含有各种抗凝剂的反应溶液中孵育，所述抗凝剂包括乙二胺四乙酸（EDTA-2Na）、肝素或酸性柠檬酸葡萄糖。在我们医院的FCIP实践中，将全外周血收集在含有EDTA-2NA作为抗凝剂的2 mL采血管中（Terumo，Tokyo，Japan; EDTA-2Na的最终浓度：1.63 mg/ml）。
CD23, a type II transmembrane glycoprotein, belongs to the C-type (Ca2þ-dependent) lectin-like superfamily. The CD23 protein is cleaved from cell surfaces as a soluble form and exerts pleiotropic activities in addition to the regulation of IgE responses and B-cell-activation and-growth [1]. CD23 is widely distributed in many normal cells, including T and B lymphocytes, monocytes, and follicular dendritic cells, and its expression is subject to different regulations by various stimuli [2, 3]. CD23 expression is a useful and pertinent diagnostic marker to differentiate between chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma (MCL), and other indolent lymphomas, and has been linked to their clinicopathological findings and prognoses [4, 5]. Thus, the precise evaluation and analysis of CD23 expression in target cells by flow-cytometric immunophenotyping (FCIP) and immunohistochemistry (IHC) are the key and critical processes for the strict diagnosis and its relevant most appropriate therapeutic choice [6, 7]. The interaction of CD23 with IgE is also a therapeutic targeting mechanism to prevent allergic diseases [8].In the clinical setting, the surface CD23 expression is evaluated in anticoagulated whole peripheral blood, bone marrow aspirates or single cell suspensions from their relevant flesh tissues, by the direct immunofluorescence staining FCIP analysis [9-12]. Therefore, primary antibodies against CD23 may be directly and simultaneously incubated with target cells in reaction solutions that contain various anticoagulants, including ethylenediaminetetraacetic acid (EDTA-2Na), heparin or acid citrate dextrose. In FCIP practice in our hospital, whole peripheral blood is collected in 2mL Vacutainer tubes containing EDTA-2NA as an anticoagulant (Terumo, Tokyo, Japan; final concentration of EDTA-2Na: 1.63 mg/
登录
查看更多内容
健康韩国淋巴细胞增多症患者中单克隆 B 细胞淋巴细胞增多症的患病率和免疫表型特征
影响因子：
4.9
作者：
I. Yoo;Sung Hoan Bang;D. Lim;S. Kim;Kyunga Kim;Hee;Sun;D. Cho
通讯作者：
D. Cho
CD40 连接后人类 B 细胞前体增殖并表达 CD23
影响因子：
15.3
作者：
S. Saeland;rie Duver;I. Moreau;J. Banchereau
通讯作者：
J. Banchereau
影响因子：
3.4
作者：
Jurisic, Vladimir;Colovic, Natasa;Colovic, Milica
通讯作者：
Colovic, Milica
滤泡性淋巴瘤中的 CD23 表达：临床病理相关性。
影响因子：
3.5
作者：
H. Olteanu;T. Fenske;A. Harrington;A. Szabo;Peng;S. Kroft
通讯作者：
S. Kroft
Epstein-Barr 病毒核抗原 2 特异性诱导 B 细胞激活抗原 CD23 的表达。
DOI：
10.1073/pnas.84.10.3452
发表时间：
1987
影响因子：
11.1
作者：
Wang,F;Gregory,CD;Rowe,M;Rickinson,AB;Wang,D;Birkenbach,M;Kikutani,H;Kishimoto,T;Kieff,E
通讯作者：
Kieff,E