The Impact of Long-term Physical Inactivity on Adipose Tissue Immunometabolism.
The Impact of Long-term Physical Inactivity on Adipose Tissue Immunometabolism.
复制标题
长期身体不活跃对脂肪组织免疫代谢的影响。
DOI：
10.1210/clinem/dgab647
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发表时间：
2022-01-01
期刊：
影响因子：
--
通讯作者：
Thompson D
中科院分区：
文献类型：
--
作者：
Trim WV;Walhin JP;Koumanov F;Bouloumié A;Lindsay MA;Travers RL;Turner JE;Thompson D
脂肪组织和缺乏体力活动都会影响代谢健康和全身炎症，但脂肪组织对慢性缺乏体力活动的反应尚不清楚。这项工作的目的是描述长期缺乏身体活动对健康年轻男性脂肪组织的影响。我们收集了20名健康年轻男性在完全卧床休息60天前后的皮下脂肪组织，减少能量摄入以维持能量平衡和脂肪量。我们使用RNA测序、流式细胞术、离体组织培养和靶蛋白分析来检查脂肪组织表型。我们的研究结果表明，当脂肪量保持稳定时，脂肪组织转录组、基质细胞区室和胰岛素信号蛋白丰度在很大程度上不受卧床休息的影响。然而，有几个脂肪因子的循环浓度增加，包括血浆瘦素，这是与无活性诱导的血浆胰岛素的增加和缺乏离体培养的脂肪组织在标准化的培养条件下。当能量摄入没有严格控制时，身体不活动引起的脂肪因子浓度（如瘦素）紊乱，而脂肪量没有变化，可能在临床设施外产生深远的代谢影响。
Adipose tissue and physical inactivity both influence metabolic health and systemic inflammation, but how adipose tissue responds to chronic physical inactivity is unknown. This work aimed to characterize the impact of chronic physical inactivity on adipose tissue in healthy, young males. We collected subcutaneous adipose tissue from 20 healthy, young men before and after 60 days of complete bed rest with energy intake reduced to maintain energy balance and fat mass. We used RNA sequencing, flow cytometry, ex vivo tissue culture, and targeted protein analyses to examine adipose tissue phenotype. Our results indicate that the adipose tissue transcriptome, stromal cellular compartment, and insulin signaling protein abundance are largely unaffected by bed rest when fat mass is kept stable. However, there was an increase in the circulating concentration of several adipokines, including plasma leptin, which was associated with inactivity-induced increases in plasma insulin and absent from adipose tissue cultured ex vivo under standardized culture conditions. Physical inactivity–induced disturbances to adipokine concentrations such as leptin, without changes to fat mass, could have profound metabolic implications outside a clinical facility when energy intake is not tightly controlled.
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影响因子：
16.2
作者：
Hojbjerre, Lise;Sonne, Mette Paulli;Stallknecht, Bente
通讯作者：
Stallknecht, Bente
影响因子：
7.7
作者：
Hojbjerre, Lise;Sonne, Mette Paulli;Stallknecht, Bente
通讯作者：
Stallknecht, Bente
影响因子：
6.5
作者：
Fu, YC;Luo, NL;Garvey, WT
通讯作者：
Garvey, WT
影响因子：
3.3
作者：
Hojbjerre, Lise;Alibegovic, Amra C.;Stallknecht, Bente
通讯作者：
Stallknecht, Bente
DOI：
10.1038/ijo.2016.238
发表时间：
2017-03
期刊：
International journal of obesity (2005)
影响因子：
--
作者：
Burnett LC;Skowronski AA;Rausch R;LeDuc CA;Leibel RL
通讯作者：
Leibel RL