Transgenic ZmMYB167 Miscanthus sinensis with increased lignin to boost bioenergy generation for the bioeconomy.
Transgenic ZmMYB167 Miscanthus sinensis with increased lignin to boost bioenergy generation for the bioeconomy.
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DOI：
10.1186/s13068-023-02279-2
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发表时间：
2023-02-22
期刊：
影响因子：
--
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中科院分区：
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芒草属多年生C4禾本科植物由于其在边际土地上的高生物量积累、低环境影响和在其生产生命期内的维护要求而被广泛认为是领先的和有前途的专用生物能源作物。迫切需要在社会政治和环境方面加大替代、负担得起和绿色生物能源的生产，并重新调整净零碳排放的轨道。因此，扩大芒属植物的种植规模，将其作为可再生能源的生物量来源，可以发挥重要作用，从战略上实现可持续发展目标，促进日益增长的生物基经济。某些芒草基因型因其在广泛范围内的生物量生产力而特别有趣。由于芳香族生物质组分木质素比细胞壁多糖具有更高的能量密度，并且通常用作发热量或发热量的指标，因此基因工程可能是开发M.本发明提供了具有增加的木质素含量的冬虫夏草生物质，从而提高生物质的能量值。为此目的，转基因M.通过农杆菌介导的转化来表达ZmMYB 167，ZmMYB 167是已知用于调节C3和C4草中木质素生物合成的MYB转录因子。获得了4个独立的转基因ZmMYB 167芒株系。转基因植株的株高、分蘖和地上部干重等农艺性状与野生型对照植株没有差异。与对照植物相比，转基因植物的总木质素含量高约15-24%。然而，在转基因植物中，结构性碳水化合物葡聚糖和木聚糖分别减少约2-7%和约8- 10%。此外，ZmMYB 167在转基因植物中的表达没有改变木质素组成、酚类化合物或酶糖化效率产量，但重要的是提高了芒草生物质的总能量水平，相当于每公顷高出10%的能量产量。这项研究强调了ZmMYB 167作为遗传木质素生物工程干预的合适目标，旨在推进和发展木质纤维素生物质供应链，以实现可再生生物能源的可持续生产。在线版本包含补充材料，可通过10.1186/s13068-023-02279-2获得。
Perennial C4 grasses from the genus Miscanthus are widely regarded as leading and promising dedicated bioenergy crops due to their high biomass accumulation on marginal land with low environmental impacts and maintenance requirements over its productive life. There is an urgent socio-political and environmental need to ramp up the production of alternative, affordable and green bioenergy sources and to re-direct the net zero carbon emissions trajectory. Hence, up-scaling of Miscanthus cultivation as a source of biomass for renewable energy could play an important role to strategically address sustainable development goals for a growing bio-based economy. Certain Miscanthus sinensis genotypes are particularly interesting for their biomass productivity across a wide range of locations. As the aromatic biomass component lignin exhibits a higher energy density than cell wall polysaccharides and is generally used as an indicator for heating or calorific value, genetic engineering could be a feasible strategy to develop M. sinensis biomass with increased lignin content and thus improving the energetic value of the biomass. For this purpose, transgenic M. sinensis were generated by Agrobacterium-mediated transformation for expression of ZmMYB167, a MYB transcription factor known for regulating lignin biosynthesis in C3 and C4 grasses. Four independent transgenic ZmMYB167 Miscanthus lines were obtained. Agronomic traits such as plant height, tillering and above-ground dry weight biomass of the transgenic plants were not different to that of wild-type control plants. Total lignin content of the transgenic plants was ~ 15–24% higher compared with control plants. However, the structural carbohydrates, glucan and xylan, were decreased by ~ 2–7% and ~ 8–10%, respectively, in the transgenic plants. Moreover, expression of ZmMYB167 in transgenic plants did not alter lignin composition, phenolic compounds or enzymatic saccharification efficiency yields but importantly improved total energy levels in Miscanthus biomass, equivalent to 10% higher energy yield per hectare. This study highlights ZmMYB167 as a suitable target for genetic lignin bioengineering interventions aimed at advancing and developing lignocellulosic biomass supply chains for sustainable production of renewable bioenergy. The online version contains supplementary material available at 10.1186/s13068-023-02279-2.
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影响因子：
4.2
作者：
da Costa RM;Lee SJ;Allison GG;Hazen SP;Winters A;Bosch M
通讯作者：
Bosch M
影响因子：
6.9
作者：
Bosch M;Mayer CD;Cookson A;Donnison IS
通讯作者：
Donnison IS
影响因子：
13.8
作者：
Bhatia R;Gallagher JA;Gomez LD;Bosch M
通讯作者：
Bosch M
PtoMYB92 是毛白杨次生细胞壁形成过程中木质素生物合成途径的转录激活剂。
影响因子：
4.9
作者：
Li, Chaofeng;Wang, Xianqiang;Luo, Keming
通讯作者：
Luo, Keming
DOI：
10.1007/978-1-0716-0621-6_16
发表时间：
2020-01-01
期刊：
PLANT CELL WALL, 2 EDITION
影响因子：
--
作者：
da Costa, Ricardo M. F.;Barrett, William;Allison, Gordon G.
通讯作者：
Allison, Gordon G.