Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles.
Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles.
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DOI：
10.1590/s1678-9946201860018
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发表时间：
2018
影响因子：
1.9
通讯作者：
Pillai S
中科院分区：
文献类型：
--
作者：
Ashmore D;Chaudhari A;Barlow B;Barlow B;Harper T;Vig K;Miller M;Singh S;Nelson E;Pillai S
由于对抗生素的耐药性，大肠杆菌可引起败血症、肠炎、食源性疾病和尿路感染等多种疾病，引起公共卫生领域的关注。银纳米颗粒(AgNP)以其生物相容性和抗菌活性而闻名，可能被证明是一种替代治疗方法，特别是作为伤口敷料。在这项研究中，我们比较了两种聚合物包裹的银纳米颗粒(含10%银(Ag10%+聚合物)或99%银(AgPVP))与普通未包覆银纳米颗粒(AgNP)的抗菌效果。利用原子力显微镜对纳米粒子进行表征，并通过最小抑菌浓度(MIC)和细菌生长曲线分析比较其抗菌效果，然后利用扫描电子显微镜(SEM)和定量逆转录聚合酶链式反应(qRT-PCR)对其进行分子研究。银纳米粒仅在0.621毫克/毫升时抑制大肠杆菌的生长，是两种包衣纳米粒所需浓度(0.312毫克/毫升)的两倍。同样，当两种包被纳米粒的浓度均为0.156 mg/mL时，细菌生长早在8h就受到了抑制，而普通银纳米粒的浓度为0.312 mg/mL。扫描电子显微镜数据显示，纳米颗粒破坏细胞膜，导致细菌细胞溶解，细胞内容物排出，部分细胞完全解体。在纳米颗粒处理的大肠杆菌中，与TCA循环相关的基因(aceF和frdB)和与氨基酸代谢相关的基因(gadB，METL，ARGC)的表达显著下调。聚合物包覆银纳米粒的银离子浓度的降低不影响其对大肠杆菌的抑菌效果。
Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) are known for their biocompatibility and antibacterial activity, and may prove to be an alternative method of treatment, especially as wound dressings. In this study, we compared the antibacterial efficacy of two polymer-coated silver nanoparticles either containing 10% Ag (Ag 10% + Polymer), or 99% Ag (AgPVP) in relation to plain uncoated silver nanoparticles (AgNP). Atomic force microscopy was used to characterize the nanoparticles, and their antibacterial efficacy was compared by the minimum inhibitory concentration (MIC) and bacterial growth curve assays, followed by molecular studies using scanning electron microscopy (SEM) and (qRT- PCR). AgNP inhibited the growth of E. coli only at 0.621 mg/mL, which was double the concentration required for both coated nanoparticles (0.312 mg/mL). Similarly, bacterial growth was impeded as early as 8 h at 0.156 mg/mL of both coated nanoparticles as compared to 0.312 mg/mL for plain AgNP. SEM data showed that nanoparticles damaged the cell membrane, resulting in bacterial cell lysis, expulsion of cellular contents, and complete disintegration of some cells. The expression of genes associated with the TCA cycle (aceF and frdB) and amino acid metabolism (gadB, metL, argC) were substantially downregulated in E. coli treated with nanoparticles. The reduction in the silver ion (Ag+) concentration of polymer-coated AgNP did not affect their antibacterial efficacy against E. coli.
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影响因子：
10.2
作者：
dos Santos CA;Jozala AF;Pessoa A Jr;Seckler MM
通讯作者：
Seckler MM
影响因子：
2
作者：
Horie, Masanori;Kato, Haruhisa;Iwahashi, Hitoshi
通讯作者：
Iwahashi, Hitoshi
DOI：
10.1007/s12154-011-0063-9
发表时间：
2011-10-01
期刊：
Journal of chemical biology
影响因子：
--
作者：
Bryaskova, Rayna;Pencheva, Daniela;Kantardjiev, Todor
通讯作者：
Kantardjiev, Todor
影响因子：
4.3
作者：
Cheng, Victor W. T.;Tran, Quang M.;Weiner, Joel H.
通讯作者：
Weiner, Joel H.
影响因子：
6.1
作者：
El-Zahry, Marwa R.;Mahmoud, Amer;Lendl, Bernhard
通讯作者：
Lendl, Bernhard