Involvement of a host Cathepsin L in symbiont-induced cell death.
Involvement of a host Cathepsin L in symbiont-induced cell death.
复制标题
DOI：
10.1002/mbo3.632
复制
发表时间：
2018-10
期刊：
影响因子：
3.4
通讯作者：
McFall-Ngai MJ
中科院分区：
文献类型：
--
作者：
Peyer SM;Kremer N;McFall-Ngai MJ
组织蛋白酶L基因的宿主鱿鱼，Euprymna candiopes，是上调在第一个小时的殖民共生弧菌fischeri。此时，共生器官开始细胞死亡介导的形态发生，仅在共生开始时才有功能。本研究的目的是确定组织蛋白酶L（一种与其他动物细胞凋亡相关的半胱氨酸蛋白酶）是否在宿主鱿鱼中共生体诱导的细胞死亡中起关键作用。序列分析和生物化学表征表明，该蛋白具有组织蛋白酶L功能所必需的关键残基和结构域，并且在这些蛋白酶的典型pH范围内具有活性。通过原位杂交和免疫细胞化学，我们将转录本和蛋白质分别定位于与费氏弧菌相互作用的细胞。该蛋白的活性发生沿着共生体定殖的路径。特异性组织蛋白酶L、非特异性半胱氨酸蛋白酶和半胱天冬酶抑制剂各自独立地在不同程度上减弱活性和细胞死亡。此外，一种特异性抗体使细胞死亡减少了约50%。这些数据共同提供了证据，证明组织蛋白酶L是共生体诱导的细胞死亡中的关键组分，其将宿主组织从定植形态转变为促进成熟缔合的形态。
The cathepsin L gene of the host squid, Euprymna scolopes, is upregulated during the first hours of colonization by the symbiont Vibrio fischeri. At this time, the symbiotic organ begins cell death‐mediated morphogenesis in tissues functional only at the onset of symbiosis. The goal of this study was to determine whether Cathepsin L, a cysteine protease associated with apoptosis in other animals, plays a critical role in symbiont‐induced cell death in the host squid. Sequence analysis and biochemical characterization demonstrated that the protein has key residues and domains essential for Cathepsin L function and that it is active within the pH range typical of these proteases. With in situ hybridization and immunocytochemistry, we localized the transcript and protein, respectively, to cells interacting with V. fischeri. Activity of the protein occurred along the path of symbiont colonization. A specific Cathepsin L, nonspecific cysteine protease, and caspase inhibitor each independently attenuated activity and cell death to varying degrees. In addition, a specific antibody decreased cell death by ~50%. Together these data provide evidence that Cathepsin L is a critical component in the symbiont‐induced cell death that transforms the host tissues from a colonization morphology to one that promotes the mature association.
登录
查看更多内容
影响因子：
6.7
作者：
Arockiaraj, Jesu;Gnanam, Annie J.;Kasi, Marimuthu
通讯作者：
Kasi, Marimuthu
DOI：
10.1086/bblv226n1p56
发表时间：
2014-02
期刊：
The Biological bulletin
影响因子：
--
作者：
Koropatnick T;Goodson MS;Heath-Heckman EA;McFall-Ngai M
通讯作者：
McFall-Ngai M
影响因子：
30.3
作者：
Kremer N;Philipp EE;Carpentier MC;Brennan CA;Kraemer L;Altura MA;Augustin R;Häsler R;Heath-Heckman EA;Peyer SM;Schwartzman J;Rader BA;Ruby EG;Rosenstiel P;McFall-Ngai MJ
通讯作者：
McFall-Ngai MJ
影响因子：
5.8
作者：
Arnold, K;Bordoli, L;Schwede, T
通讯作者：
Schwede, T
影响因子：
4.5
作者：
GABBOTT, PA;PEEK, K
通讯作者：
PEEK, K