Carotenoid biosynthesis is associated with low-temperature adaptation in Rhodosporidium kratochvilovae.
Carotenoid biosynthesis is associated with low-temperature adaptation in Rhodosporidium kratochvilovae.
复制标题
DOI：
10.1186/s12866-022-02728-2
复制
发表时间：
2022-12-24
期刊：
影响因子：
4.2
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
--
作者：
Guo, Rui;Liu, Tao;Guo, Caina;Chen, Gongshui;Fan, Jingdie;Zhang, Qi
关键词：
低温极大地限制了微生物的生长。微生物的低温适应涉及多种机制。类胡萝卜素是天然存在的脂溶性色素，可作为抗氧化剂，保护细胞和组织免受自由基和单线态氧的有害影响。然而，关于微生物低温下类胡萝卜素生物合成调控的研究有限。在本研究中，我们研究了红孢子虫 kratochvilovae YM25235 冷适应菌株中类胡萝卜素与低温适应之间的相关性。使用已建立的基于内源性 U6 启动子的 CRISPR/Cas9 基因编辑系统，通过敲除双功能番茄红素环化酶/八氢番茄红素合酶基因 (RKCrtYB)，抑制 YM25235 中的类胡萝卜素生物合成。用丙酮提取类胡萝卜素，采用分光光度法和高效液相色谱法分析类胡萝卜素的含量和组成。然后，在低温下测定YM25235中的活性氧（ROS）水平和生长速率。结果表明，YM25235菌株在低温下类胡萝卜素生物合成和ROS水平增加，抑制类胡萝卜素生物合成与ROS水平升高和YM25235低温生长速率显着降低有关。 YM25235 类胡萝卜素生物合成的调节与低温适应有关。我们的研究结果为进一步研究YM25235适应低温胁迫的机制提供了坚实的基础。在线版本包含可在 10.1186/s12866-022-02728-2 获取的补充材料。
Low temperatures greatly limit the growth of microorganisms. Low-temperature adaptation in microorganisms involves multiple mechanisms. Carotenoids are naturally occurring lipid-soluble pigments that act as antioxidants and protect cells and tissues from the harmful effects of free radicals and singlet oxygen. However, studies on the regulation of carotenoid biosynthesis at low temperatures in microorganisms are limited. In this study, we investigated the correlation between carotenoids and low-temperature adaptation in the cold-adapted strain of Rhodosporidium kratochvilovae YM25235. Carotenoid biosynthesis in YM25235 was inhibited by knocking out the bifunctional lycopene cyclase/phytoene synthase gene (RKCrtYB) using the established CRISPR/Cas9 gene-editing system based on endogenous U6 promoters. The carotenoids were extracted with acetone, and the content and composition of the carotenoids were analyzed by spectrophotometry and HPLC. Then, the levels of reactive oxygen species (ROS) and the growth rate in YM25235 were determined at a low temperature. The results indicated that the carotenoid biosynthesis and ROS levels were increased in the YM25235 strain at a low temperature and inhibition of carotenoid biosynthesis was associated with higher ROS levels and a significant decrease in the growth rate of YM25235 at a low temperature. The regulation of carotenoid biosynthesis was associated with low-temperature adaptation in YM25235. Our findings provided a strong foundation for conducting further studies on the mechanism by which YM25235 can adapt to low-temperature stress. The online version contains supplementary material available at 10.1186/s12866-022-02728-2.
登录
查看更多内容
影响因子：
64.5
作者：
Hsu PD;Lander ES;Zhang F
通讯作者：
Zhang F
影响因子：
17.3
作者：
Gaj, Thomas;Gersbach, Charles A.;Barbas, Carlos F., III
通讯作者：
Barbas, Carlos F., III
影响因子：
2.5
作者：
Avalos, Javier;Carmen Limon, M.
通讯作者：
Carmen Limon, M.
影响因子：
2.4
作者：
Bhosale, P;Gadre, RV
通讯作者：
Gadre, RV
影响因子：
2.8
作者：
Jagannadham, MV;Chattopadhyay, MK;Shivaji, S
通讯作者：
Shivaji, S