Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice.
Transcriptome analysis of grain-filling caryopses reveals involvement of multiple regulatory pathways in chalky grain formation in rice.
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DOI：
10.1186/1471-2164-11-730
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发表时间：
2010-12-30
期刊：
影响因子：
4.4
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Liu X;Guo T;Wan X;Wang H;Zhu M;Li A;Su N;Shen Y;Mao B;Zhai H;Mao L;Wan J
稻米胚乳垩白是稻米的一个品种特性，它不仅影响稻米的外观和碾磨特性，而且影响稻米的蒸煮质构和适口性。然而，稻米垩白是一个复杂的数量遗传性状，其形成的分子机制还知之甚少。对高垩白近等基因系CSSL 50 -1与其正常亲本Asominori的胚乳垩白进行了比较。成熟籽粒的理化分析表明，与Asominori相比，CSSL 50 -1含有较高水平的直链淀粉和8 DP短链支链淀粉，但较低的中长12 DP支链淀粉。对15个开花后第二天的颖果进行转录组分析，共鉴定出623个差异表达基因（P < 0.01），其中上调基因324个，下调基因299个。这些基因可分为18大类，其中65.3%属于6大功能群：信号转导、细胞拯救/防御、转录、蛋白质降解、糖代谢和氧化还原稳态。详细的途径解剖表明，参与蔗糖和淀粉合成的基因上调，而那些参与非淀粉多糖下调。研究发现，参与氧化还原稳态的几个基因在CSSL 50 -1中也有较高的表达水平，表明ROS在籽粒垩白形成中的潜在作用。在稻米垩白形成过程中检测到广泛的基因表达变化。这些差异表达的基因中有一半以上涉及几个重要的基因类别，包括信号转导、转录、碳水化合物代谢和氧化还原稳态，表明垩白形成涉及多种代谢和调控途径。
Grain endosperm chalkiness of rice is a varietal characteristic that negatively affects not only the appearance and milling properties but also the cooking texture and palatability of cooked rice. However, grain chalkiness is a complex quantitative genetic trait and the molecular mechanisms underlying its formation are poorly understood. A near-isogenic line CSSL50-1 with high chalkiness was compared with its normal parental line Asominori for grain endosperm chalkiness. Physico-biochemical analyses of ripened grains showed that, compared with Asominori, CSSL50-1 contains higher levels of amylose and 8 DP (degree of polymerization) short-chain amylopectin, but lower medium length 12 DP amylopectin. Transcriptome analysis of 15 DAF (day after flowering) caryopses of the isogenic lines identified 623 differential expressed genes (P < 0.01), among which 324 genes are up-regulated and 299 down-regulated. These genes were classified into 18 major categories, with 65.3% of them belong to six major functional groups: signal transduction, cell rescue/defense, transcription, protein degradation, carbohydrate metabolism and redox homeostasis. Detailed pathway dissection demonstrated that genes involved in sucrose and starch synthesis are up-regulated, whereas those involved in non-starch polysaccharides are down regulated. Several genes involved in oxidoreductive homeostasis were found to have higher expression levels in CSSL50-1 as well, suggesting potential roles of ROS in grain chalkiness formation. Extensive gene expression changes were detected during rice grain chalkiness formation. Over half of these differentially expressed genes are implicated in several important categories of genes, including signal transduction, transcription, carbohydrate metabolism and redox homeostasis, suggesting that chalkiness formation involves multiple metabolic and regulatory pathways.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
4.4
作者：
Cheng C;Yun KY;Ressom HW;Mohanty B;Bajic VB;Jia Y;Yun SJ;de los Reyes BG
通讯作者：
de los Reyes BG
影响因子：
6.9
作者：
Gorantla, Markandeya;Babu, P. R.;Reddy, Arjula R.
通讯作者：
Reddy, Arjula R.
影响因子：
7.4
作者：
Fujita, N;Yoshida, M;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
3.1
作者：
Li, JM;Xiao, JH;McCouch, SR
通讯作者：
McCouch, SR