Differentially expressed genes in the head of the 2nd instar pre-molting larvae of the nm2 mutant of the silkworm, Bombyx mori.
Differentially expressed genes in the head of the 2nd instar pre-molting larvae of the nm2 mutant of the silkworm, Bombyx mori.
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家蚕 nm2 突变体 2 龄蜕皮前幼虫头部差异表达基因。
DOI：
10.1371/journal.pone.0180160
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Wang P;Bi S;Wu F;Xu P;Shen X;Zhao Q
蜕皮是家蚕幼虫期一个重要的生理过程，受多种激素和多肽的控制。2龄不蜕皮表型的家蚕突变体（nm2）在2龄不能蜕皮，7天后死亡。nm2突变体的蜕皮素滴度低于野生型，可以通过20E和胆固醇喂养来挽救突变体。定位克隆结果表明，nm2突变体的表型与BmCPG10的结构改变有关。为了探究BmCPG10与脱皮激素滴度之间的可能关系以及受BmCPG10影响的基因，我们以野生型菌株C603为对照，对nm2突变体进行了数字基因表达（DGE）谱分析。结果发现1727个差异表达基因，其中651个基因在nm2中上调，1076个基因下调。BLASTGO分析表明，这些差异表达基因参与了多种生物过程、细胞成分和分子功能。KEGG分析显示，这些差异表达基因在240个途径中富集，包括代谢途径、胰腺分泌途径、蛋白质消化吸收途径、脂肪消化吸收途径和甘油脂代谢途径。为了验证DGE结果的准确性，我们进行了定量反转录PCR (qRT-PCR)，重点研究了几个相关通路中的关键基因，结果与DGE结果高度一致。我们发现表皮蛋白基因、蜕皮激素生物合成基因和蜕皮激素相关核受体基因存在显著差异，而幼体激素和几丁质生物合成基因无显著差异。本研究结果为进一步研究nm2突变体的形成机制奠定了基础。
Molting is an important physiological process in the larval stage of Bombyx mori and is controlled by various hormones and peptides. The silkworm mutant that exhibits the phenotype of non-molting in the 2nd instar (nm2) is incapable of molting in the 2nd instar and dies after seven or more days. The ecdysone titer in the nm2 mutant is lower than that in the wildtype, and the mutant can be rescued by feeding with 20E and cholesterol. The results of positional cloning indicated that structural alteration of BmCPG10 is responsible for the phenotype of the nm2 mutant. To explore the possible relationship between BmCPG10 and the ecdysone titer as well as the genes affected by BmCPG10, digital gene expression (DGE) profile analysis was conducted in the nm2 mutant, with the wildtype strain C603 serving as the control. The results revealed 1727 differentially expressed genes, among which 651 genes were upregulated and 1076 were downregulated in nm2. BLASTGO analysis showed that these differentially expressed genes were involved in various biological processes, cellular components and molecular functions. KEGG analysis indicated an enrichment of these differentially expressed genes in 240 pathways, including metabolic pathways, pancreatic secretion, protein digestion and absorption, fat digestion and absorption and glycerolipid metabolism. To verify the accuracy of the DGE results, quantitative reverse transcription PCR (qRT-PCR) was performed, focusing on key genes in several related pathways, and the results were highly consistent with the DGE results. Our findings indicated significant differences in cuticular protein genes, ecdysone biosynthesis genes and ecdysone-related nuclear receptors genes, but no significant difference in juvenile hormone and chitin biosynthesis genes was detected. Our research findings lay the foundation for further research on the formation mechanism of the nm2 mutant.
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影响因子：
2.7
作者：
ASHBURNER, M
通讯作者：
ASHBURNER, M
影响因子：
4.6
作者：
Enya S;Ameku T;Igarashi F;Iga M;Kataoka H;Shinoda T;Niwa R
通讯作者：
Niwa R
家蚕核受体：深入了解基因组结构和发育表达
影响因子：
3.8
作者：
Cheng, Daojun;Xia, Qingyou;Xiang, Zhonghuai
通讯作者：
Xiang, Zhonghuai
影响因子：
2.2
作者：
Ali, Md. Saheb;Rahman, R. F.;Swapon, Ahsanul Haque
通讯作者：
Swapon, Ahsanul Haque
影响因子：
4.1
作者：
Cohen, E
通讯作者：
Cohen, E