A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila.
A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila.
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DOI：
10.1038/srep06586
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发表时间：
2014-10-10
影响因子：
4.6
通讯作者：
Niwa R
中科院分区：
文献类型：
--
作者：
Enya S;Ameku T;Igarashi F;Iga M;Kataoka H;Shinoda T;Niwa R
在昆虫中，蜕皮和变态的精确时间是严格由蜕皮类固醇指导的，蜕皮类固醇是由前胸腺（PG）中的膳食胆固醇合成的。在过去的十年中，已经鉴定和表征了几种蜕皮类固醇生成酶，其中一些是由万圣节基因编码的。在这里，我们报告了一种新的万圣节基因，noppera-bo（nobo），编码谷胱甘肽S-转移酶家族的一员。nobo被鉴定为主要在果蝇Drosophilamelanogaster的PG中表达的基因。我们产生了一个nobo基因敲除突变体，显示胚胎致死性和裸露的角质层结构。这些表型是典型的万圣节突变体显示胚胎蜕皮激素缺乏症。此外，PG特异性nobo敲除幼虫显示出停滞的表型和降低的20-羟基蜕皮激素（20 E）滴度。重要的是，胚胎和幼虫的表型都被20 E或胆固醇的施用所拯救。我们还证实，PG细胞在nobo功能丧失的幼虫异常积累胆固醇。考虑到胆固醇是PG中蜕皮甾体生物合成的最上游材料，我们的研究结果提高了nobo在调节昆虫类固醇生物合成中胆固醇行为中起关键作用的可能性。
In insects, the precise timing of moulting and metamorphosis is strictly guided by ecdysteroids that are synthesised from dietary cholesterol in the prothoracic gland (PG). In the past decade, several ecdysteroidogenic enzymes, some of which are encoded by the Halloween genes, have been identified and characterised. Here, we report a novel Halloween gene, noppera-bo (nobo), that encodes a member of the glutathione S-transferase family. nobo was identified as a gene that is predominantly expressed in the PG of the fruit fly Drosophila melanogaster. We generated a nobo knock-out mutant, which displayed embryonic lethality and a naked cuticle structure. These phenotypes are typical for Halloween mutants showing embryonic ecdysteroid deficiency. In addition, the PG-specific nobo knock-down larvae displayed an arrested phenotype and reduced 20-hydroxyecdysone (20E) titres. Importantly, both embryonic and larval phenotypes were rescued by the administration of 20E or cholesterol. We also confirm that PG cells in nobo loss-of-function larvae abnormally accumulate cholesterol. Considering that cholesterol is the most upstream material for ecdysteroid biosynthesis in the PG, our results raise the possibility that nobo plays a crucial role in regulating the behaviour of cholesterol in steroid biosynthesis in insects.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
11.8
作者：
McBrayer, Zofeyah;Ono, Hajime;O'Connor, Michael B.
通讯作者：
O'Connor, Michael B.
DOI：
10.1007/978-90-481-8622-8_1
发表时间：
2010-01-01
期刊：
CHOLESTEROL BINDING AND CHOLESTEROL TRANSPORT PROTEINS: STRUCTURE AND FUNCTION IN HEALTH AND DISEASE
影响因子：
--
作者：
Gimpl, Gerald
通讯作者：
Gimpl, Gerald
影响因子：
11.4
作者：
FOLEY, KP;LEONARD, MW;ENGEL, JD
通讯作者：
ENGEL, JD
影响因子：
4.1
作者：
Kim, Jaekwang;Suh, Hyunsuk;Yim, Jeongbin
通讯作者：
Yim, Jeongbin