Imaging mass spectrometry of diversified cardiolipin molecular species in the brain.
Imaging mass spectrometry of diversified cardiolipin molecular species in the brain.
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DOI：
10.1021/ac5011876
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发表时间：
2014-07-01
影响因子：
7.4
通讯作者：
Kagan, V. E.
中科院分区：
文献类型：
--
作者：
Amoscato, A. A.;Sparvero, L. J.;He, R. R.;Watkins, S.;Bayir, H.;Kagan, V. E.
MALDI成像质谱（MALDI-IMS）已成功地用于映射组织切片中的不同脂质，但现有的协议无法检测到大脑中的细胞和线粒体生理学所必需的多种独特的心磷脂（CL）。我们已经开发出能够对脑组织中的单个CL进行成像的方法。这是通过消除离子抑制作用来实现的，所述离子抑制作用通过（i）用1-乙基-3-[3-（二甲氨基）丙基]-碳二亚胺盐酸盐交联组织上的含羧基/氨基的分子和（ii）通过磷脂酶C处理去除高度丰富的磷脂酰胆碱头部基团。这些处理允许在100 μm分辨率下检测CL物质，并且不影响脑组织CL的量或分子物质分布。当与增强矩阵应用程序相结合时，这些修改允许在大脑的各个区域（包括丘脑、海马和皮质）中对多种CL物质进行可视化和映射。齿状核和放射层等区域的CL信号比海马结构内的其他区域高。缰核（Hb）/背侧第三脑室（D3 V）和侧脑室（LV）区域被确定为CL“热点”。我们的方法还允许结构MS/MS碎片和映射的CL与确定的脂肪酸残基，并证明了一个非随机分布的个人氧化（含多不饱和脂肪酸）和非氧化（非多不饱和）CL在不同的解剖区域的大脑。据我们所知，该方法是第一种用于脑组织中多种CL分子定位的无标记方法。
MALDI imaging mass spectrometry (MALDI-IMS) has been used successfully in mapping different lipids in tissue sections, yet existing protocols fail to detect the diverse species of mitochondria-unique cardiolipins (CLs) in the brain which are essential for cellular and mitochondrial physiology. We have developed methods enabling the imaging of individual CLs in brain tissue. This was achieved by eliminating ion suppressive effects by (i) cross-linking carboxyl/amino containing molecules on tissue with 1-ethyl-3-[3-(dimethylamino)propyl]-carbodiimide hydrochloride and (ii) removing highly abundant phosphatidylcholine head groups via phospholipase C treatment. These treatments allowed the detection of CL species at 100 μm resolution and did not affect the amount or molecular species distribution of brain tissue CLs. When combined with augmented matrix application, these modifications allowed the visualization and mapping of multiple CL species in various regions of the brain including the thalamus, hippocampus, and cortex. Areas such as the dentate and stratum radiatum exhibited higher CL signals than other areas within the hippocampal formation. The habenular nuclear (Hb)/dorsal third ventricle (D3 V) and lateral ventricle (LV) areas were identified as CL “hot spots”. Our method also allowed structural MS/MS fragmentation and mapping of CLs with identified fatty acid residues and demonstrated a nonrandom distribution of individual oxidizable (polyunsaturated fatty acid containing) and nonoxidizable (nonpolyunsaturated containing) CLs in different anatomical areas of the brain. To our knowledge, this method is the first label-free approach for molecular mapping of diversified CLs in brain tissue.
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DOI：
10.1007/s13361-011-0122-z
发表时间：
2011-06
影响因子：
3.2
作者：
Hankin JA;Farias SE;Barkley RM;Heidenreich K;Frey LC;Hamazaki K;Kim HY;Murphy RC
通讯作者：
Murphy RC
脑神经毒剂的分子显微镜：说明它们在海马细胞层中的分布。
影响因子：
5
作者：
Colsch, Benoit;Jackson, Shelley N.;Dutta, Sucharita;Woods, Amina S.
通讯作者：
Woods, Amina S.
影响因子：
14.8
作者：
Kagan, VE;Tyurin, VA;Borisenko, GG
通讯作者：
Borisenko, GG
影响因子：
7.4
作者：
Kagan, Valerian E.;Bayir, Huelya A.;Belikova, Natalia A.;Kapralov, Olexandr;Tyurina, Yulia Y.;Tyurin, Vladimir A.;Jiang, Jianfei;Stoyanovsky, Detcho A.;Wipf, Peter;Kochanek, Patrick M.;Greenberger, Joel S.;Pitt, Bruce;Shvedova, Anna A.;Borisenko, Grigory
通讯作者：
Borisenko, Grigory
影响因子：
7.4
作者：
Angel, Peggi M.;Spraggins, Jeffrey M.;Baldwin, H. Scott;Caprioli, Richard
通讯作者：
Caprioli, Richard