Comprehensive transcriptional landscape of porcine cardiac and skeletal muscles reveals differences of aging.
Comprehensive transcriptional landscape of porcine cardiac and skeletal muscles reveals differences of aging.
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DOI：
10.18632/oncotarget.23290
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发表时间：
2018-01-05
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Chen J;Zou Q;Lv D;Wei Y;Raza MA;Chen Y;Li P;Xi X;Xu H;Wen A;Zhu L;Tang G;Li M;Jiang A;Liu Y;Fu Y;Jiang Y;Li X
衰老显着影响心肌（CM）和骨骼肌（SM）。由于CM和SM的衰老过程可能不同，因此利用不同年龄条件下的CM和SM进行高通量RNA测序，以评估信使RNA（mRNA）、长链非编码RNA（lncRNA）、微小RNA（miRNA）和环状RNA（circRNA）的表达谱。几种 mRNA、lncRNA 和 miRNA 在两个年龄的两种肌肉组织之一中均高表达且一致出现。基因本体（GO）注释描述了这些基因是维持CM和SM组织正常生物学功能所必需的。此外，26个mRNA、4个lncRNA、22个miRNA和26个circRNA在心肌衰老过程中存在差异表达。此外，81个mRNA、5个lncRNA、79个miRNA和62个circRNA在骨骼肌衰老过程中存在差异表达。当比较衰老过程中CM和SM的表达谱时，发现CM和SM的衰老过程有根本的不同。此外，我们评估了肌肉衰老中的多组协作控制关系并构建了 circRNA-miRNA-mRNA 共表达网络。总之，我们的研究结果将有助于理解肌肉衰老，并为未来研究肌肉衰老的分子机制奠定基础。
Aging significantly affects the cardiac muscle (CM) and skeletal muscles (SM). Since the aging process of CM and SM may be different, high throughput RNA sequencing was performed using CM and SM in different age conditions to evaluate the expression profiles of messenger RNA (mRNA), long non-coding RNA (lncRNA), micro RNA (miRNA), and circular (circRNA). Several mRNAs, lncRNAs, and miRNAs were highly expressed and consistently appeared in both ages in one of the two muscle tissues. Gene ontology (GO) annotation described that these genes were required for maintaining normal biological functions of CM and SM tissues. Furthermore, 26 mRNAs, 4 lncRNAs, 22 miRNAs, and 26 circRNAs were differentially expressed during cardiac muscle aging. Moreover, 81 mRNAs, 5 lncRNAs, 79 miRNAs, and 62 circRNAs were differentially expressed during aging of skeletal muscle. When comparing the expression profiles of CM and SM during aging, the senescence process in CM and SM was found to be fundamentally different. In addition, we assessed multi-group cooperative control relationships and constructed circRNA-miRNA-mRNA co-expression networks in muscular aging. In conclusion, our findings will contribute to the understanding of muscular aging and provide a foundation for future studies on the molecular mechanisms underlying muscular aging.
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影响因子：
3.4
作者：
Barry G;Guennewig B;Fung S;Kaczorowski D;Weickert CS
通讯作者：
Weickert CS
影响因子：
14.9
作者：
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通讯作者：
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DOI：
10.1016/j.jocd.2015.04.012
发表时间：
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期刊：
Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry
影响因子：
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作者：
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通讯作者：
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影响因子：
4.6
作者：
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通讯作者：
Dong S
影响因子：
30.8
作者：
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通讯作者：
Salvayre, Robert