Application of the skin and bone integrated pylon with titanium oxide nanotubes and seeded with dermal fibroblasts.
Application of the skin and bone integrated pylon with titanium oxide nanotubes and seeded with dermal fibroblasts.
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DOI：
10.1177/0309364614550261
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发表时间：
2015-12
影响因子：
1.5
通讯作者：
Pitkin M
中科院分区：
文献类型：
--
作者：
Shevtsov MA;Yudintceva N;Blinova M;Pinaev G;Galibin O;Potokin I;Popat KC;Pitkin M
在动物模型中体外和体内研究了纳米管皮肤和骨整合塔（SBIP）骨植入的可行性和安全性。直接肢体假体的直接骨骼附着与高发生率的经皮感染和髓管内固定装置的松动有关，促使仔细评估各种增强皮肤-装置和骨-装置界面的方法。 SBIP系统构成了之前评估的不同修改的技术平台。目前的研究评估了纳米治疗 SBIP 与真皮成纤维细胞预接种的组合。我们假设，与单独进行纳米处理和成纤维细胞接种相比，这种组合将增强细胞与 SBIP 的相互作用。在SBIP上制备TiO2纳米管，并在植入前在塔上培养取自兔子皮肤的成纤维细胞。体外实验表明，具有纳米管表面的样品中的细胞密度高于用作对照的未修饰的塔。在 6 个月的观察期内，所有动物均未出现术后并发症。随后从兔子股骨中提取的塔的扫描电镜显示，与检测到斑片状纤维血管向内生长的对照样品相比，塔和软组织之间的接触稳定。这一令人鼓舞的结果促使人们进一步研究接种真皮成纤维细胞的纳米结构 SBIP 系统的综合特性及其临床应用的优化。
The feasibility and safety of in bone implantation of the skin and bone integrated pylons (SBIP) with nanotubes was investigated in vitro and in vivo in the animal model. Direct Direct skeletal attachment of limb prostheses is associated with high rate of transcutaneous infection and loosening of the fixture in the medullary canal prompting for careful assessment of various means for enhancing the skin-device and bone-device interface. The SBIP system constitutes a technological platform for different modifications being evaluated previously. The current study assessed the combination of nano treatment SBIP with its pre seeding with dermal fibroblasts. We hypothesized that this combination will enhance cell interaction with SBIP compared to nano treatment and the fibroblast seeding when done separately. TiO2 nanotubes were fabricated on the SBIP, and the fibroblasts taken from rabbit's skin were cultured on the pylons before implantation. The in vitro experiments demonstrated higher cellular density in the samples with a nanotubular surface than in the non modified pylons used as control. There were no postoperative complications in any of the animals during the 6 month observation period. Subsequent SEM of the pylon extracted from the rabbit's femur showed the stable contact between the pylon and soft tissues in comparison to control samples where the patchy fibrovascular ingrowth was detected. The promising results prompt further investigation of the integrative properties of the nanotextured SBIP system seeded with dermal fibroblasts and its optimization for clinical application.
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影响因子：
1.8
作者：
Drygas, Kevin A.;Taylor, Robert;McAlexander, Heather
通讯作者：
McAlexander, Heather
DOI：
10.1682/jrrd.2006.12.0160
发表时间：
2007-01-01
影响因子：
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作者：
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DOI：
10.1682/jrrd.2012.11.0202
发表时间：
2013-01-01
影响因子：
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作者：
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通讯作者：
Rousse, Serge D.
影响因子：
4.9
作者：
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通讯作者：
Pitkin, Mark