Characteristic Distribution of Ciguatoxins in the Edible Parts of a Grouper, Variola louti.
Characteristic Distribution of Ciguatoxins in the Edible Parts of a Grouper, Variola louti.
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DOI：
10.3390/toxins13030218
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发表时间：
2021-03-17
期刊：
影响因子：
4.2
通讯作者：
Yasumoto T
中科院分区：
文献类型：
--
作者：
Oshiro N;Nagasawa H;Kuniyoshi K;Kobayashi N;Sugita-Konishi Y;Asakura H;Yasumoto T
雪卡鱼中毒（CFP）是最常见的海鲜中毒综合症之一；它是由食用受雪卡毒素（CTX）污染的海洋鱼类引起的。大多数 CFP 病例是由食用鱼肉引起的，但人们存在一种传统观念，认为鱼头和内脏毒性更大，应避免食用。与内脏不同，支持头部具有高毒性这一传说的科学数据很少。我们从来自日本冲绳的 5 只黄边琴尾鱼 (Variola louti) 个体的鱼片、头部和眼睛中制备了组织样本，并通过 LC-MS/MS 分析了 CTX。确认了三种CTX，即CTX1B、52-epi-54-deoxyCTX1B和54-deoxyCTX1B，其比例相似。毒素几乎均匀地分布在与鱼片和鱼头分开准备的肉中。在同一个体样本中，分别测试的鱼片中的肉和鱼头的肉具有相同的毒素水平和成分。因此，我们得出结论，用于 LC-MS/MS 分析的肉样本可以从身体的任何部位采集。然而，眼球周围组织的 CTX 水平比肉中高出两到四倍。本研究首次提供了证明眼睛具有高毒性的科学数据。
Ciguatera fish poisoning (CFP) is one of the most frequently encountered seafood poisoning syndromes; it is caused by the consumption of marine finfish contaminated with ciguatoxins (CTXs). The majority of CFP cases result from eating fish flesh, but a traditional belief exists among people that the head and viscera are more toxic and should be avoided. Unlike the viscera, scientific data to support the legendary high toxicity of the head is scarce. We prepared tissue samples from the fillet, head, and eyes taken from five yellow-edged lyretail (Variola louti) individuals sourced from Okinawa, Japan, and analyzed the CTXs by LC-MS/MS. Three CTXs, namely, CTX1B, 52-epi-54-deoxyCTX1B, and 54-deoxyCTX1B, were confirmed in similar proportions. The toxins were distributed nearly evenly in the flesh, prepared separately from the fillet and head. Within the same individual specimen, the flesh in the fillet and the flesh from the head, tested separately, had the same level and composition of toxins. We, therefore, conclude that flesh samples for LC-MS/MS analysis can be taken from any part of the body. However, the tissue surrounding the eyeball displayed CTX levels two to four times higher than those of the flesh. The present study is the first to provide scientific data demonstrating the high toxicity of the eyes.
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影响因子：
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