The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.
The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.
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四尿草履虫 pawn-B 的克隆及分子分析。
DOI：
10.1093/genetics/155.3.1105
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Kung,C
中科院分区：
文献类型：
--
作者：
Haynes,WJ;Ling,KY;Preston,RR;Saimi,Y;Kung,C
Pawn mutants ofParamecium tetraurelialack a depolarization-activated Ca2+current and do not swim backward. Using the method of microinjection and sorting a genomic library, we have cloned a DNA fragment that complements pawn-B (pwB/pwB). The minimal complementing fragment is a 798-bp open reading frame (ORF) that restores the Ca2+current and the backward swimming when expressed. This ORF contains a 29-bp intron and is transcribed and translated. The translated product has two putative transmembrane domains but no clear matches in current databases. Mutations in the availablepwBalleles were found within this ORF. Thed4-95andd4-96alleles are single base substitutions, whiled4-662(previously pawn-D) harbors a 44-bp insertion that matches an internal eliminated sequence (IES) found in the wild-type germline DNA except for a single C-to-T transition. Northern hybridizations and RT-PCR indicate that d4-662 transcripts are rapidly degraded or not produced. A second 155-bp IES in the wild-type germline ORF excises at two alternative sites spanning three asparagine codons. ThepwBORF appears to be separated from a 5′ neighboring ORF by only 36 bp. The close proximity of the two ORFs and the location of the pwB protein as indicated by GFP-fusion constructs are discussed.
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影响因子：
3.3
作者：
K. Hatakeyama;T. Takasaki;M. Watanabe;K. Hinata
通讯作者：
K. Hatakeyama;T. Takasaki;M. Watanabe;K. Hinata
通过草履虫中的互补克隆基因。
DOI：
10.3109/01677069609107064
发表时间：
1996
期刊：
Journal of neurogenetics.
影响因子：
--
作者：
Haynes,WJ;Ling,KY;Saimi,Y;Kung,C
通讯作者：
Kung,C
四尿草履虫中补充 Ca2 通道突变的细胞质因子的表征。
影响因子：
1.9
作者：
N. Haga;M. Forte;Y. Saimi;C. Kung
通讯作者：
C. Kung
用于草履虫互补克隆的索引基因组文库
影响因子：
2.2
作者：
A. Keller;Jean Cohen
通讯作者：
Jean Cohen
影响因子：
64.8
作者：
D. Oertel;S. Schein;C. Kung
通讯作者：
C. Kung