Genome-Wide Investigation of the Phospholipase C Gene Family in Zea mays.
Genome-Wide Investigation of the Phospholipase C Gene Family in Zea mays.
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玉米磷脂酶 C 基因家族的全基因组研究
DOI：
10.3389/fgene.2020.611414
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发表时间：
2020
影响因子：
3.7
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Zhu J;Zhou Y;Li J;Li H
磷脂酶C（PLC）是肌醇磷脂代谢的主要水解酶之一，在植物生长发育和逆境胁迫的多种信号转导过程中发挥重要作用。虽然许多植物PLC的特性已被研究，但玉米PLC基因尚未得到全面鉴定。根据这项研究，在玉米中鉴定了5个磷脂酰肌醇特异性PLC（PI-PLC）和6个非特异性PLC（NPC）基因。玉米的PI-PLC和NPC基因与其它植物的同源基因相比，在进化关系、蛋白质序列、保守基序和基因结构等方面都是保守的。ZmPLC-GFP融合蛋白在拟南芥原生质体细胞中的瞬时表达表明ZmPLCs具有多定位性。转录水平分析表明，ZmPLC在不同组织和非生物胁迫下存在显著差异。关联分析表明，部分ZmPLC与508份玉米自交系的农艺性状显著相关。这些结果有助于深入研究ZmPLC的功能，并为上级玉米品种的产量和品质的调控提供良好的候选靶标。
Phospholipase C (PLC) is one of the main hydrolytic enzymes in the metabolism of phosphoinositide and plays an important role in a variety of signal transduction processes responding to plant growth, development, and stress. Although the characteristics of many plant PLCs have been studied, PLC genes of maize have not been comprehensively identified. According to the study, five phosphatidylinositol-specific PLC (PI-PLC) and six non-specific PLC (NPC) genes were identified in maize. The PI-PLC and NPC genes of maize are conserved compared with homologous genes in other plants, especially in evolutionary relationship, protein sequences, conserved motifs, and gene structures. Transient expression of ZmPLC-GFP fusion protein in Arabidopsis protoplast cells showed that ZmPLCs are multi-localization. Analyses of transcription levels showed that ZmPLCs were significantly different under various different tissues and abiotic stresses. Association analysis shown that some ZmPLCs significantly associated with agronomic traits in 508 maize inbred lines. These results contribute to study the function of ZmPLCs and to provide good candidate targets for the yield and quality of superior maize cultivars.
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影响因子：
6.9
作者：
Kocourková D;Krcková Z;Pejchar P;Veselková S;Valentová O;Wimalasekera R;Scherer GF;Martinec J
通讯作者：
Martinec J
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
4.3
作者：
Di Fino, Luciano M.;D'Ambrosio, Juan Martin;Laxalt, Ana M.
通讯作者：
Laxalt, Ana M.
影响因子：
7.3
作者：
Peters, Carlotta;Kim, Sang-Chul;Wang, Xuemin
通讯作者：
Wang, Xuemin
在非生物胁迫、激素处理和组织类型中验证玉米 qPCR 的潜在参考基因
影响因子：
3.7
作者：
Lin Y;Zhang C;Lan H;Gao S;Liu H;Liu J;Cao M;Pan G;Rong T;Zhang S
通讯作者：
Zhang S