VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.
VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.
复制标题
VvBAP1 参与葡萄的耐冷性。
DOI：
10.3389/fpls.2018.00726
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Hou L;Zhang G;Zhao F;Zhu D;Fan X;Zhang Z;Liu X
大多数商业葡萄品种起源于欧洲葡萄。虽然这些品种具有优良的感官品质，但它们在冬季对寒冷的耐受性相对较差，导致葡萄藤受到严重损害。因此，低温是制约葡萄产业进一步发展的瓶颈之一。因此，对葡萄耐冷机理的研究是十分重要的。BON结合蛋白1 （BAP1）是最近发现的一种磷脂结合蛋白。在拟南芥中，AtBAP1的表达可以通过低温调控；然而，BAP1在葡萄藤中的功能尚未见报道。我们之前在葡萄中克隆了VvBAP1基因，生物信息学分析表明它含有保守的钙依赖性C2蛋白结构域。然而，人们对其功能和机制知之甚少。在本研究中，对抗寒葡萄品种“F-242”和“左右红”以及对寒冷敏感的葡萄品种“赤霞珠”和“霞多丽”进行了冷处理。在抗寒品种中，VvBAP1的表达量显著高于冷敏感品种，表明VvBAP1可能与葡萄的冷响应过程有关。以抗寒葡萄品种‘F-242’为材料，在4°C和CaCl2处理下，通过qRT-PCR检测VvBAP1的相对表达量。低温和低温Ca2+信号均可诱导VvBAP1表达。此外，我们克隆了VvBAP1基因并将其转移到拟南芥中，获得了过表达VvBAP1的植株。对低温处理（4℃和-8℃）的植株进行生化分析和基因表达分析。结果表明，转基因植株的超氧化物歧化酶和过氧化物酶活性均高于野生型植株，细胞膜通透性和丙二醛含量均低于野生型植株。可溶性糖含量和糖代谢相关基因BAM4-7、SS4、G6PD5的表达量均显著高于WT。此外，CBF1、CBF3、COR15a、COR6.6、COR27、KIN1等低温应答信号基因的表达也有所增强。综上所述，VvBAP1可通过调控葡萄糖含量和激活抗氧化酶活性来增强葡萄的抗寒性。
The majority of commercial grape cultivars originate from the European grape. While these cultivars have excellent organoleptic qualities, they suffer from a relatively poor tolerance to the cold experienced during winter, resulting in significant damage to grapevines. Thus, low temperature is one of the bottlenecks that restrict the further growth of the grape industry. Research on the mechanism of cold tolerance in grapes is therefore very important. BON association protein 1 (BAP1) is a recently discovered phospholipid-binding protein. In Arabidopsis, the expression of AtBAP1 can be regulated via low temperature; however, the function of BAP1 in the grapevine has not been reported. The VvBAP1 gene was cloned in our previous studies in grapes, and bioinformatics analysis showed that it harbors the conservative calcium-dependent C2 protein domain. However, little is known about its function and underlying mechanism. In this study, cold treatment was applied to the cold-resistant grape varieties ‘F-242’ and ‘Zuoyouhong’ as well as to the cold-sensitive grape varieties ‘Cabernet Sauvignon’ and ‘Chardonnay.’ The expression level of VvBAP1 in the cold-resistant varieties was significantly higher than in the cold-sensitive varieties, indicating that VvBAP1 could be associated with the cold response processes in the grapevine. Using the cold-resistant grape variety ‘F-242’ as material, with the 4°C and CaCl2 treatment, the relative expression of VvBAP1 was determined via qRT-PCR. Both low temperature and low-temperature signal Ca2+ induced VvBAP1 expression. In addition, the VvBAP1 gene was cloned and transferred into Arabidopsis to generate VvBAP1 overexpressing plants. Biochemical assays and gene expression analyses were conducted on plants subjected to low temperature treatments (4 and -8°C). The obtained results showed that the activities of superoxide dismutase and peroxidase in these transgenic plants were higher than those in wild type (WT) plants, and that cell membrane permeability and malondialdehyde content were both lower compared to WT plants. Furthermore, the content of soluble sugars and the expression levels of sugar-metabolizing related genes, such as BAM4-7, SS4, and G6PD5, were significantly higher than those of WT plants. Furthermore, the expression of low temperature response signal genes, including CBF1, CBF3, COR15a, COR6.6, COR27, and KIN1, were also enhanced. In summary, these results showed that VvBAP1 could strengthen the cold resistance in the grapevine through adjusting and controlling the sugar content and activating antioxidant enzyme activity.
登录
查看更多内容
乙烯通过调节乙烯响应因子 057 的表达来积极调节葡萄的耐冷性
影响因子：
4.6
作者：
Sun X;Zhao T;Gan S;Ren X;Fang L;Karungo SK;Wang Y;Chen L;Li S;Xin H
通讯作者：
Xin H
影响因子：
5.3
作者：
Hochberg U;Degu A;Toubiana D;Gendler T;Nikoloski Z;Rachmilevitch S;Fait A
通讯作者：
Fait A
影响因子：
4.2
作者：
Dubrovina, Alexandra S.;Kiselev, Konstantin V.;Aleynova, Olga A.
通讯作者：
Aleynova, Olga A.
影响因子：
1
作者：
Fu, Peining;Wang, Wenjie;Liu, Xin
通讯作者：
Liu, Xin
影响因子：
5.2
作者：
Sicher, Richard
通讯作者：
Sicher, Richard