Transcription of detoxification genes after permethrin selection in the mosquito Aedes aegypti.
Transcription of detoxification genes after permethrin selection in the mosquito Aedes aegypti.
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DOI：
10.1111/j.1365-2583.2011.01113.x
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发表时间：
2012-02
影响因子：
2.6
通讯作者：
Black WC 4th
中科院分区：
文献类型：
--
作者：
Saavedra-Rodriguez K;Suarez AF;Salas IF;Strode C;Ranson H;Hemingway J;Black WC 4th
在五代氯氰菊酯实验室选择之前、期间和之后，监测了六个埃及伊蚊菌株的基因表达变化：五个来自梅西科尤卡坦半岛的F2-F3样本和一个来自伊基托斯的F2样本，Perú。每个菌株产生三个生物复制系。根据致死和击倒氯氰菊酯浓度(LC_(50)、K_(50))以及电压门控钠通道(PARA)基因Ile1016替换频率的变化来衡量对选择的反应。用“伊蚊解毒”基因芯片检测290个代谢解毒基因的表达变化。选择同时提高了Lc50、KC50和Ile1016频率。Ile1016频率与差异转录基因的数量呈负相关。伊基托斯菌株缺乏Ile1016等位基因，经选择后有51个基因被差异转录，而墨西哥菌株为10-18个基因。几乎没有相同的基因在野生株之间差异转录，但10个细胞色素P450基因在多个品系中上调。二氯氰菊酯在室内的室内适应。埃及伊蚊在遗传上很复杂，很大程度上受到地理来源和副基因中先前存在的靶点不敏感的影响。对人工选择作出反应的基因缺乏一致性，以及它们的反应方向存在差异，这挑战了一个或几个基因控制二氯苯菊酯代谢抗性的假设。试图确定一个或几个可预测与氯菊酯适应相关的代谢基因可能是徒劳的。
Changes in gene expression before, during and after five generations of permethrin laboratory selection were monitored in six strains of Aedes aegypti: five F2 – F3 collections from the Yucatán Peninsula of México and one F2 from Iquitos, Perú. Three biological replicate lines were generated for each strain. The response to selection was measured as changes in the lethal and knockdown permethrin concentrations (LC50, KC50) and in the frequency of the Ile1,016 substitution in the voltage gated sodium channel (para) gene. Changes in expression of 290 metabolic detoxification genes were measured using the “Aedes Detox” microarray. Selection simultaneously increased the LC50, KC50 and Ile1,016 frequency. There was an inverse relationship between Ile1,016 frequency and the numbers of differentially transcribed genes. The Iquitos strain lacked the Ile1,016 allele and 51 genes were differentially transcribed following selection as compared to 10–18 genes in the Mexican strains. Very few of the same genes were differentially transcribed among field strains but ten cytochrome P450 genes were upregulated in more than one strain. Laboratory adaptation to permethrin in Ae. aegypti is genetically complex and largely conditioned by geographic origin and preexisting target site insensitivity in the para gene. The lack of uniformity in the genes that responded to artificial selection as well as differences in the direction of their responses challenges the assumption that one or a few genes control permethrin metabolic resistance. Attempts to identify one or a few metabolic genes that are predictably associated with permethrin adaptation may be futile.
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影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
0.4
作者：
Flores, Adriana E.;Reyes Solis, Guadalupe;Ponce Garcia, Gustavo
通讯作者：
Ponce Garcia, Gustavo
影响因子：
2.6
作者：
Poupardin, R.;Riaz, M. A.;Reynaud, S.
通讯作者：
Reynaud, S.
影响因子：
3.8
作者：
Poupardin, Rodolphe;Reynaud, Stephane;David, Jean-Philippe
通讯作者：
David, Jean-Philippe
DOI：
10.1590/s0074-02762005000400017
发表时间：
2005-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
da-Cunha, Marcella Pereira;Lima, José Bento Pereira;Valle, Denise
通讯作者：
Valle, Denise