Effects of dietary scFOS and lactobacilli on survival, growth, and disease resistance of hybrid tilapia
Effects of dietary scFOS and lactobacilli on survival, growth, and disease resistance of hybrid tilapia
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饲料中的短链果糖和乳酸菌对杂交罗非鱼存活、生长和抗病性的影响
DOI：
10.1016/j.aquaculture.2016.12.013
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发表时间：
2017-03
期刊：
影响因子：
4.5
通讯作者：
Zhigang Zhou
中科院分区：
文献类型：
--
作者：
Chao Ran;Suxu He;Yalin Yang;Zhigang Zhou
本研究旨在筛选肠道黏附乳酸杆菌菌株，并研究scFOS对罗非鱼幼鱼生长性能、肠道黏附菌群及抗病性的影响。首先，采用rpoBPCR-DGGE技术研究了乳酸杆菌菌株在罗非鱼肠道中的粘附特性，以及scFOS对乳酸杆菌粘附的影响。在基础饲料中添加等量的7株乳酸菌（2.0 × 107个/g），连续饲喂2周，然后分别饲喂基础饲料和scFOS饲料4周。结果表明，菌株JCM 1170和JCM 1149的粘附力最强，scFOS增加了它们的相对丰度。在另一个实验中，给鱼喂食含有108个细胞/g饲料的JCM 1170或JCM 1149（有或没有scFOS）的饮食5周。在不同的处理中，没有观察到存活率、增重或饲料转化率的显著差异。454焦磷酸测序结果表明，肠道中有10个优势菌门，其中变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、梭菌门（Fusobacteria）和放线菌门（Actinobacteria）是最主要的类群。肠道粘附菌群的组成受益生菌/合生元的影响。攻毒试验表明，合生元JCM 1149和scFOS对鱼体的抗黄曲霉能力有显著保护作用（P< 0.05）。因此，可推荐108 cell/g饲料的L.plantarumJCM1149和1 mg/g scFOS作为罗非鱼的合生元配方。
The present study was aimed to select gut adhesiveLactobacillusstrains and evaluate the combined effects with scFOS on the growth performance, gut adhesive bacteria and disease resistance of juvenile hybrid tilapia. Firstly, the adhesive property ofLactobacillusstrains in the gut of tilapia, and effect of scFOS on the adhesion ofLactobacilluswere determined byrpoBPCR-DGGE. Fish were fed with diet supplemented with seven probioticLactobacillusstrains at equal amounts (2.0 × 107cells/g basal diet) for 2 weeks, followed by basal or scFOS supplemented diet for 4 weeks. Results showed that strain JCM 1170 and JCM1149 was the most highly-adhesive strains and scFOS increased their relative abundance. In another experiment, fish were fed a diet containing 108cells/g feed of JCM1170 OR JCM1149 with or without scFOS for 5 weeks. No significant differences in survival rate, weight gain, or feed conversion were observed in the different treatments. 10 predominant phyla were identified from gut samples by 454 pyrosequencing, and Proteobacteria, Firmicutes, Fusobacteria and Actinobacteria were the most predominant group. The composition of gut adherent microbiota was affected by probiotics/synbiotics. The challenge test showed that synbiotics of JCM 1149 and scFOS protected fish againstA.hydrophila(P< 0.05). In conclusion,L.plantarumJCM 1149 at 108cell/g feed and scFOS at 1 mg/g may be recommended as a synbiotics formulation for tilapia.
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DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
T. Ph
通讯作者：
T. Ph
影响因子：
3.5
作者：
Akrami, R.;Nasri-Tajan, M.;Jafarpour, S. A.
通讯作者：
Jafarpour, S. A.
影响因子：
4.7
作者：
Giri, Sib Sankar;Sukumaran, V.;Oviya, M.
通讯作者：
Oviya, M.
饲喂两种不同乳酸菌菌株的杂交罗非鱼幼鱼粘附肠道细菌组成、免疫力和抗病性的比较
影响因子：
4.7
作者：
Liu, Wenshu;Ren, Pengfei;Zhou, Zhigang
通讯作者：
Zhou, Zhigang
影响因子：
4.5
作者：
He, Suxu;Zhou, Zhigang;Yoon, Ilkyu
通讯作者：
Yoon, Ilkyu