The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.
The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.
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家蚕（Bombyx mori）细胞凋亡的基因组基础。
DOI：
10.1186/1471-2164-11-611
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发表时间：
2010-10-31
期刊：
影响因子：
4.4
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Zhang JY;Pan MH;Sun ZY;Huang SJ;Yu ZS;Liu D;Zhao DH;Lu C
细胞凋亡受一系列基因的有序调控，在生长发育、免疫应答等重要生理过程中发挥重要作用，近年来，在人、果蝇、线虫等模型动物中进行了大量的研究。然而，缺乏家蚕的基因组数据限制了其在细胞凋亡研究中的有用性，尽管家蚕作为鳞翅目昆虫的代表和有效的模型系统的优点。在此，我们已经确定了家蚕中的寄生虫病相关基因，并将它们与昆虫，哺乳动物和线虫的基因进行了比较。从新组装的基因组数据库中，在全基因组范围内分析家蚕中与烟草赤星病相关的基因，使用核苷酸和蛋白质Blast搜索。共鉴定出52个凋亡相关候选基因，包括5个caspase家族成员、2个肿瘤坏死因子（TNF）超家族成员、1个Bcl-2家族成员、4个杆状病毒IAP（inhibitor of apoptosis）repeat（BIR）结构域家族成员和1个RHG（Reaper，Hid，Grim，and Sickle; Drosophila cell death activators）家族成员。此外，我们确定了一个新的caspase家族成员，BmCaspase-New，BmDronc的两个剪接变体，和Bm 3585，哺乳动物TNF超家族成员同源物。使用从BmE-SWU 1细胞中分离的cDNA模板克隆并测序了其中的23个凋亡相关基因。序列分析表明，这些基因可能在细胞凋亡中起关键作用。家蚕具有潜在的抗病相关基因。我们推测，经典的内在和外在的凋亡途径可能是活跃的家蚕。这些结果为进一步研究家蚕寄生虫病的相关机理奠定了基础。
Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on. Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes. From the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle; Drosophila cell death activators) family member. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Sequence analyses revealed that these genes could have key roles in apoptosis. Bombyx mori possesses potential apoptosis-related genes. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. These results lay the foundation for further apoptosis-related study in Bombyx mori.
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影响因子：
4.1
作者：
Iga, Masatoshi;Iwami, Masafumi;Sakurai, Sho
通讯作者：
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影响因子：
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作者：
Katsuma, Susumu;Mita, Kazuei;Shimada, Toru
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Shimada, Toru
影响因子：
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作者：
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影响因子：
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通讯作者：
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作者：
BUTTERWORTH, FM;BODENSTEIN, D;KING, RC
通讯作者：
KING, RC