The Borrelia burgdorferi CheY3 response regulator is essential for chemotaxis and completion of its natural infection cycle.
The Borrelia burgdorferi CheY3 response regulator is essential for chemotaxis and completion of its natural infection cycle.
复制标题
DOI：
10.1111/cmi.12617
复制
发表时间：
2016-12
影响因子：
3.4
通讯作者：
Motaleb MA
中科院分区：
文献类型：
--
作者：
Novak EA;Sekar P;Xu H;Moon KH;Manne A;Wooten RM;Motaleb MA
伯氏疏螺旋体具有复杂的趋化系统，但该生物体如何利用这一系统在其自然的地方性生活周期知之甚少。在B. burgdorferi，我们发现只有cheY 3的缺失导致运动性改变和趋化性表型显著降低。虽然Δ cheY 3在未进食的蜱中保持正常密度，但与亲本或cheY 3互补的螺旋体相比，它们在进食的蜱中的数量显著减少。重要的是，用Δ cheY 3感染的蜱喂养的小鼠没有发生持续性感染。活体共聚焦显微镜分析发现，Δ cheY 3螺旋体是能动的，但似乎不能逆转方向，并执行由亲本菌株显示的特征性向后-向前运动。随后，Δ cheY 3在接种后数天内被“捕获”在皮肤基质中，在注射后96小时内从皮肤针接种部位清除，并且不扩散到远处组织。有趣的是，尽管Δ cheY 3细胞在注射后96小时内被清除，但这种减弱的感染引起显著水平的B。伯氏特异性IgM和IgG。总之，这些数据表明，cheY 3介导的趋化性是至关重要的运动，传播，和小鼠和蜱内和之间的螺旋体的活力。
Borrelia burgdorferi possesses a sophisticated and complex chemotaxis system but how the organism utilizes this system in its natural enzootic life cycle is poorly understood. Of the three CheY chemotaxis response regulators in B. burgdorferi, we found that only deletion of cheY3 resulted in an altered motility and significantly reduced chemotaxis phenotype. Though ΔcheY3 maintained normal densities in unfed ticks, their numbers were significantly reduced in fed ticks compared to the parental or cheY3-complemented spirochetes. Importantly, mice fed upon by the ΔcheY3-infected ticks did not develop a persistent infection. Intravital confocal microscopy analyses discovered that the ΔcheY3 spirochetes were motile, but appeared unable to reverse direction and perform the characteristic backward-forward motility displayed by the parental strain. Subsequently, the ΔcheY3 became “trapped” in the skin matrix within days of inoculation, were cleared from the skin needle-inoculation site within 96 hours post-injection, and did not disseminate to distant tissues. Interestingly, although ΔcheY3 cells were cleared within 96 hours post-injection, this attenuated infection elicited significant levels of B. burgdorferi-specific IgM and IgG. Taken together, these data demonstrate that cheY3-mediated chemotaxis is crucial for motility, dissemination, and viability of the spirochete both within and between mice and ticks.
登录
查看更多内容
影响因子：
5.7
作者：
Hajdušek O;Síma R;Ayllón N;Jalovecká M;Perner J;de la Fuente J;Kopáček P
通讯作者：
Kopáček P
影响因子：
3.1
作者：
Brown, JP;Zachary, JF;Wooten, RM
通讯作者：
Wooten, RM
影响因子：
6.4
作者：
FIKRIG, E;BOCKENSTEDT, LK;FLAVELL, RA
通讯作者：
FLAVELL, RA
影响因子：
4.4
作者：
Fikrig, E;Feng, W;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
3.1
作者：
Hagman, KE;Yang, XF;Norgard, MV
通讯作者：
Norgard, MV