In vivo MR imaging of intraarterially delivered magnetically labeled mesenchymal stem cells in a canine stroke model.
In vivo MR imaging of intraarterially delivered magnetically labeled mesenchymal stem cells in a canine stroke model.
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犬中风模型中动脉内输送的磁性标记间充质干细胞的体内 MR 成像。
DOI：
10.1371/journal.pone.0054963
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shi HB
中科院分区：
文献类型：
--
作者：
Lu SS;Liu S;Zu QQ;Xu XQ;Yu J;Wang JW;Zhang Y;Shi HB
本研究旨在评估超顺磁性氧化铁（SPIO）标记的间充质干细胞（MSCs）在犬中风模型中的动脉内（IA）传递和体内磁共振成像的可行性。用自制的SPIO标记从小猎犬骨髓中获取的间充质干细胞。成年beagle犬（n = 12）采用自体血栓闭塞左大脑中动脉近端（MCA），然后用5根French椎导管闭塞左颈内动脉（ICA） 2小时。1周后，移植前将犬分为3组：A组：MCA完全再通，B组：MCA不完全再通，C组：无MCA再通。3×106标记的间充质干细胞通过左ICA传递。细胞移植前、移植后1小时和24小时、移植后每周至第4周，连续进行体内MRI成像。将24小时和4周时间点的MRI结果与组织学结果进行比较。自制的SPIO可用于标记间充质干细胞，且不影响细胞活力。A组间充质干细胞广泛分布于左脑半球，B组移植细胞较少，移植后1小时C组未见细胞。细胞移植当天梗死越大，脑内移植细胞越多。移植的间充质干细胞在4周内可通过MRI有效追踪，普鲁士蓝染色可在梗死周围区发现。骨髓间充质干细胞移植于犬脑卒中模型是可行的。移植前同侧MCA状况和梗死体积都可能影响靶脑移植细胞的数量。体内MR成像可用于跟踪SPIO标记后IA传递的MSCs。
This study aimed to evaluate the feasibility of intraarterial (IA) delivery and in vivo MR imaging of superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) in a canine stroke model. MSCs harvested from beagles’ bone marrow were labeled with home-synthesized SPIO. Adult beagle dogs (n = 12) were subjected to left proximal middle cerebral artery (MCA) occlusion by autologous thrombus, followed by two-hour left internal carotid artery (ICA) occlusion with 5 French vertebral catheter. One week later, dogs were classified as three groups before transplantation: group A: complete MCA recanalization, group B: incomplete MCA recanalization, group C: no MCA recanalization. 3×106 labeled-MSCs were delivered through left ICA. Series in vivo MRI images were obtained before cell grafting, one and 24 hours after transplantation and weekly thereafter until four weeks. MRI findings were compared with histological studies at the time point of 24 hours and four weeks. Home-synthesized SPIO was useful to label MSCs without cell viability compromise. MSCs scattered widely in the left cerebral hemisphere in group A, while fewer grafted cells were observed in group B and no cell was detected in group C at one hour after transplantation. A larger infarction on the day of cell transplantation was associated with more grafted cells in the brain. Grafted MSCs could be tracked effectively by MRI within four weeks and were found in peri-infarction area by Prussian blue staining. It is feasible of IA MSCs transplantation in a canine stroke model. Both the ipsilateral MCA condition and infarction volume before transplantation may affect the amount of grafted cells in target brain. In vivo MR imaging is useful for tracking IA delivered MSCs after SPIO labeling.
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