microRNAs of parasitic helminths - Identification, characterization and potential as drug targets.
microRNAs of parasitic helminths - Identification, characterization and potential as drug targets.
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DOI：
10.1016/j.ijpddr.2014.03.001
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发表时间：
2014-08
影响因子：
4
通讯作者：
Devaney, Eileen
中科院分区：
文献类型：
--
作者：
Britton, Collette;Winter, Alan D.;Gillan, Victoria;Devaney, Eileen
本文综述了microRNA在蠕虫转录后基因调控中的重要性。越来越多的蠕虫miRNA数据可从深度测序中获得。一些miRNA是蠕虫特异性的，许多是每个物种的新发现。miRNAs可以调节寄生虫和宿主基因的表达。miRNA抑制剂和模拟物的摄取对于功能分析是可行的。microRNA（miRNAs）是一类参与转录后基因调控的非编码小RNA。它们首先在自由生活的线虫秀丽隐杆线虫中被发现，其中miRNA lin-4和let-7被证明对于调节正确的发育进程至关重要。后来发现let-7的序列在高等生物中是保守的，let-7以及其他miRNA表达的变化与某些癌症相关，表明了重要的调节作用。一些miRNAs已被证明具有重要的功能，但许多miRNAs的作用目前尚不清楚。随着基因组序列数据的日益可用性，现在已经通过小RNA文库的深度测序和生物信息学方法从许多寄生蠕虫中鉴定出miRNA。虽然一些miRNA在一系列生物体中广泛保守，但另一些则是蠕虫特异性的，并且许多对于每个物种来说都是新颖的。本文综述了miRNAs在蠕虫发育调控、与宿主环境相互作用及耐药性形成中的作用。使用荧光标记的小RNA证明了寄生虫的摄取，至少在体外。因此，miRNA抑制剂或模拟物的递送具有改变miRNA活性的潜力，为探索miRNA的作用提供了有用的工具，并为寄生虫控制的治疗提供了新的途径。
Importance of microRNAs in helminth post-transcriptional gene regulation is reviewed. Increasing helminth miRNA data are available from deep sequencing. Some miRNAs are helminth-specific, many are novel to each species. miRNAs may regulate parasite and host gene expression. Uptake of miRNA inhibitors and mimics is feasible for functional analysis. microRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation. They were first identified in the free-living nematode Caenorhabditis elegans, where the miRNAs lin-4 and let-7 were shown to be essential for regulating correct developmental progression. The sequence of let-7 was subsequently found to be conserved in higher organisms and changes in expression of let-7, as well as other miRNAs, are associated with certain cancers, indicating important regulatory roles. Some miRNAs have been shown to have essential functions, but the roles of many are currently unknown. With the increasing availability of genome sequence data, miRNAs have now been identified from a number of parasitic helminths, by deep sequencing of small RNA libraries and bioinformatic approaches. While some miRNAs are widely conserved in a range of organisms, others are helminth-specific and many are novel to each species. Here we review the potential roles of miRNAs in regulating helminth development, in interacting with the host environment and in development of drug resistance. Use of fluorescently-labeled small RNAs demonstrates uptake by parasites, at least in vitro. Therefore delivery of miRNA inhibitors or mimics has potential to alter miRNA activity, providing a useful tool for probing the roles of miRNAs and suggesting novel routes to therapeutics for parasite control.
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影响因子：
30.8
作者：
Desjardins, Christopher A.;Cerqueira, Gustavo C.;Goldberg, Jonathan M.;Hotopp, Julie C. Dunning;Haas, Brian J.;Zucker, Jeremy;Ribeiro, Jose M. C.;Saif, Sakina;Levin, Joshua Z.;Fan, Lin;Zeng, Qiandong;Russ, Carsten;Wortman, Jennifer R.;Fink, Doran L.;Birren, Bruce W.;Nutman, Thomas B.
通讯作者：
Nutman, Thomas B.
影响因子：
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作者：
de Wit, Elzo;Linsen, Sam E. V.;Berezikov, Eugene
通讯作者：
Berezikov, Eugene
影响因子：
2.1
作者：
Chen, Mu-Xin;Ai, Lin;Chen, Jia-Xu
通讯作者：
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影响因子：
7.5
作者：
Chekulaeva, Marina;Filipowicz, Witold
通讯作者：
Filipowicz, Witold
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.