Allele-specific marker development and selection efficiencies for both flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase genes in soybean subgenus soja.
Allele-specific marker development and selection efficiencies for both flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase genes in soybean subgenus soja.
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DOI：
10.1007/s00122-013-2063-3
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发表时间：
2013-06
影响因子：
5.4
通讯作者：
Qiu, Li-Juan
中科院分区：
文献类型：
--
作者：
Guo, Yong;Qiu, Li-Juan
颜色是大豆研究中最常用的表型标记之一。梅尔）遗传、分子和生物化学过程。两种P450依赖性单加氧酶，类黄酮3′-羟化酶（F3′H; EC1.14.3.21）和类黄酮3′，5 ′-羟化酶（F3′5′H，EC1.14.13.88），都催化类黄酮中B环的羟基化，在着色中起重要作用。以往的研究表明，大豆中的T位点是一个编码F3′H的基因，而W1位点与一个编码F3′5′H的基因共分离。这两个基因座已被确定控制种皮、花和毛的颜色。然而，F3 'H和F3' 5 'H基因在大豆中的等位基因分布尚不清楚。在这项研究中，发现了三个新的等位基因（GmF 3 ′H的四个等位基因中的两个和GmF 3 ′5′H的三个等位基因中的一个）。基于所有7个等位基因的序列多样性开发并验证了一组基因标记标记。利用这些标记对170份栽培大豆（G. max）和102个野生大豆（G.大豆）。对F3′H和F3′5′H两个亲本，分别测定了对毛色和花色的标记选择效率。结果表明，一个GmF 3 'H等位基因解释了黄褐色变异的92.2%，两个gmf 3' h等位基因解释了灰色毛色变异的63.8%。此外，两个GmF 3 ′5′H等位基因和一个gmF 3 ′5′h等位基因分别解释了紫色花和白色花的94.0%和75.3%的变异。通过这两个位点的组合，确定了种皮颜色。总的来说，90.9%的同时具有gmf 3 ′h-b和gmf 3 ′5′h等位基因的材料具有黄色种皮。因此，种皮颜色受两个以上的基因座控制。本文的在线版本（doi：10.1007/s 00122 -013-2063-3）包含补充材料，可供授权用户使用。
Color is one of the phenotypic markers mostly used to study soybean (Glycine max L. Merr.) genetic, molecular and biochemical processes. Two P450-dependent mono-oxygenases, flavonoid 3′-hydroxylase (F3′H; EC1.14.3.21) and flavonoid 3′,5′-hydroxylase (F3′5′H, EC1.14.13.88), both catalyzing the hydroxylation of the B-ring in flavonoids, play an important role in coloration. Previous studies showed that the T locus was a gene encoding F3′H and the W1 locus co-segregated with a gene encoding F3′5′H in soybean. These two genetic loci have identified to control seed coat, flower and pubescence colors. However, the allelic distributions of both F3′H and F3′5′H genes in soybean were unknown. In this study, three novel alleles were identified (two of four alleles for GmF3′H and one of three alleles for GmF3′5′H). A set of gene-tagged markers was developed and verified based on the sequence diversity of all seven alleles. Furthermore, the markers were used to analyze soybean accessions including 170 cultivated soybeans (G. max) from a mini core collection and 102 wild soybeans (G. soja). For both F3′H and F3′5′H, the marker selection efficiencies for pubescence color and flower color were determined. The results showed that one GmF3′H allele explained 92.2 % of the variation in tawny and two gmf3′h alleles explained 63.8 % of the variation in gray pubescence colors. In addition, two GmF3′5′H alleles and one gmF3′5′h allele explained 94.0 % of the variation in purple and 75.3 % in white flowers, respectively. By the combination of the two loci, seed coat color was determined. In total, 90.9 % of accessions possessing both the gmf3′h-b and gmf3′5′h alleles had yellow seed coats. Therefore, seed coat colors are controlled by more than two loci. The online version of this article (doi:10.1007/s00122-013-2063-3) contains supplementary material, which is available to authorized users.
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