Analysis of gut microbiota revealed Lactococcus garviaeae could be an indicative of skin ulceration syndrome in farmed sea cucumber Apostichopus japonicus
Analysis of gut microbiota revealed Lactococcus garviaeae could be an indicative of skin ulceration syndrome in farmed sea cucumber Apostichopus japonicus
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肠道微生物群分析显示，加维亚乳球菌可能是养殖刺参皮肤溃疡综合征的一个指标
DOI：
10.1016/j.fsi.2018.06.001
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发表时间：
2018-06
影响因子：
4.7
通讯作者：
Li Chenghua
中科院分区：
文献类型：
--
作者：
Zhang Zhen;Xing Ronglian;Lv Zhimeng;Shao Yina;Zhang Weiwei;Zhao Xuelin;Li Chenghua
累积的证据支持肠道微生物群在塑造各种动物宿主健康方面的关键作用。然而，肠道微生物群与海参疾病之间的关系知之甚少。利用Illumina测序技术，研究了健康和患皮肤溃疡综合征（SUS）的仿刺参（Apostichopus）肠道细菌群落的差异。结果表明，两个类群的细菌类群在门水平上关系密切，变形菌门占优势（>90%）。而厚壁菌门和疣微菌门在两组中呈现相反的趋势，患病组厚壁菌门丰度较高，疣微菌门丰度较低。进一步的KEGG富集显示，细菌介导的感染性疾病和信号转导途径在SUS组中被显著诱导。我们还确定了一个OTU的加尔维乳球菌厚壁菌门表现出显着不同的丰度相比，健康的受试者在患病海参。患病组和健康组的相对丰度分别为27.67% ± 10.52%和2.78% ± 2.59%。L. qPCR检测结果表明，与健康海参相比，患病海参中的gavieae基因被显著诱导（P< 0.05）。所有的结果都支持L.因此，Garviea可能是SUS暴发的潜在病原，可作为该病监测的生物指示物。
Accumulative evidence has supported the pivotal roles of gut microbiota in shaping host health in a wide range of animals. However, the relationship between gut microbiota and sea cucumber disease is poorly understood. Using the Illumina sequencing of bacterial 16 S rRNA gene, we investigated the divergence of gut bacterial communities between healthy and skin ulceration syndrome (SUS) diseased Apostichopus japonicus. The results showed that bacterial phylotypes in both groups were closely related at phylum level with predominant component of Proteobacteria (>90%). However, Firmicutes and Verrucomicrobia displayed opposite trends in two groups with higher abundance of Firmicutes and lower of Verrucomicrobia in diseased group. Further KEGG enrichment revealed that bacterial-mediated infectious diseases and signal transduction pathways were significantly induced in the SUS group. We also identified one OTU of Lactococcus garvieae from Firmicutes exhibited significantly different abundances in diseased sea cucumber as compared to healthy subjects. The relative abundance of the species was 27.67% ± 10.52% in diseased group compared to 2.78% ± 2.59% in healthy sea cucumber. Three virulence genes ofhlyⅢ,fbpandpvaencoded byL. garvieaewere investigated by qPCR, and were found to be significantly induced (P< 0.05) in diseased sea cucumbers as compared to healthy ones. All our results supported thatL. garvieaemight be a potential pathogen for SUS outbreak and could be served as a bio-indicator for this disease monitoring.
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DOI：
--
发表时间：
2006
期刊：
Journal of Dalian Fisheries University
影响因子：
--
作者：
Ma Yue-xin;Chang Yaqing;Zhang En-peng;Zhou Wei;Song Lin-sheng
通讯作者：
Ma Yue-xin;Chang Yaqing;Zhang En-peng;Zhou Wei;Song Lin-sheng
影响因子：
4.4
作者：
Ortiz-Pineda PA;Ramírez-Gómez F;Pérez-Ortiz J;González-Díaz S;Santiago-De Jesús F;Hernández-Pasos J;Del Valle-Avila C;Rojas-Cartagena C;Suárez-Castillo EC;Tossas K;Méndez-Merced AT;Roig-López JL;Ortiz-Zuazaga H;García-Arrarás JE
通讯作者：
García-Arrarás JE
影响因子：
5.4
作者：
M. Sorbara;S. Girardin
通讯作者：
M. Sorbara;S. Girardin
影响因子：
1.5
作者：
Yujie Sha;Mei Liu;Baojie Wang;Keyong Jiang;Guoqiong Sun;Lei Wang
通讯作者：
Yujie Sha;Mei Liu;Baojie Wang;Keyong Jiang;Guoqiong Sun;Lei Wang
影响因子：
24.5
作者：
C. P. Tamboli;Christel Neut;Pierre Desreumaux;J. Colombel
通讯作者：
C. P. Tamboli;Christel Neut;Pierre Desreumaux;J. Colombel