Nitrite-producing oral microbiome in adults and children.
Nitrite-producing oral microbiome in adults and children.
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DOI：
10.1038/s41598-020-73479-1
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发表时间：
2020-10-06
影响因子：
4.6
通讯作者：
Takahashi N
中科院分区：
文献类型：
--
作者：
Sato-Suzuki Y;Washio J;Wicaksono DP;Sato T;Fukumoto S;Takahashi N
最近，有人提出，口腔细菌从NO3-产生的亚硝酸盐（NO2-）可能有助于口腔和全身健康。因此，我们的目的是澄清有关口腔生物膜中细菌NO2-生产的详细信息。收集牙菌斑和舌苔样品，然后测量NO2产生活性。此外，使用含Griess试剂的琼脂覆盖法和分子生物学方法鉴定了NO2−产生菌群的组成。每毫克湿重的NO2−生成活性因个体而异，但在牙菌斑中较高。此外，厌氧菌显示出更多的NO2−产生细菌，除了在成人的牙菌斑。NO2−产生菌的比例也因个体而异，但NO2−产生活性与NO2−产生菌的数量之间存在正相关关系，特别是在牙菌斑中。总的来说，主要的NO2−产生细菌被确定为放线菌，Schaalia，韦荣氏球菌和奈瑟氏球菌。此外，Rothia在儿童的舌苔中被特异性地检测到。这些结果表明，牙菌斑具有较高的NO2−生成活性，并且该活性不取决于特定细菌的存在或细菌组成，而是取决于NO2−生成细菌的数量，尽管检测到个体间差异。
Recently, it was suggested that the nitrite (NO2−) produced from NO3− by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2−-producing bacterial population were identified using the Griess reagent-containing agar overlay method and molecular biological method. NO2−-producing activity per mg wet weight varied among individuals but was higher in dental plaque. Additionally, anaerobic bacteria exhibited higher numbers of NO2−-producing bacteria, except in the adults’ dental plaque. The proportion of NO2−-producing bacteria also varied among individuals, but a positive correlation was found between NO2−-producing activity and the number of NO2−-producing bacteria, especially in dental plaque. Overall, the major NO2−-producing bacteria were identified as Actinomyces, Schaalia, Veillonella and Neisseria. Furthermore, Rothia was specifically detected in the tongue coatings of children. These results suggest that dental plaque has higher NO2−-producing activity and that this activity depends not on the presence of specific bacteria or the bacterial compositions, but on the number of NO2−-producing bacteria, although interindividual differences were detected.
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影响因子：
3.9
作者：
Kanady, Jesica A.;Aruni, A. Wilson;Blood, Arlin B.
通讯作者：
Blood, Arlin B.
影响因子：
4.6
作者：
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影响因子：
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作者：
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Reis, Veronica Massena
影响因子：
7.4
作者：
Vanhatalo A;Blackwell JR;L'Heureux JE;Williams DW;Smith A;van der Giezen M;Winyard PG;Kelly J;Jones AM
通讯作者：
Jones AM
DOI：
10.1099/00207713-51-3-1099
发表时间：
2001-05-01
影响因子：
2.8
作者：
Coenye, T;Laevens, S;Vandamme, P
通讯作者：
Vandamme, P