Requirement for an Otopetrin-like protein for acid taste in Drosophila.
Requirement for an Otopetrin-like protein for acid taste in Drosophila.
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DOI：
10.1073/pnas.2110641118
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发表时间：
2021-12-21
影响因子：
11.1
通讯作者：
Montell C
中科院分区：
文献类型：
--
作者：
Ganguly A;Chandel A;Turner H;Wang S;Liman ER;Montell C
酸的味道对动物的生存至关重要，因为它使它们能够区分潜在的危险和有营养的食物。由于生存对酸味的普遍需求，我们测试了在酸味中起作用的受体机制可能在进化上保守的想法。在这里，我们证明了突变的果蝇基因，Otopetrin样A（OtopLA），编码一种蛋白质远亲最近发现的哺乳动物酸性味觉受体，OTOP 1，是必不可少的强烈排斥高酸性食物和温和的吸引力低酸度。温和的吸引力和强烈的厌恶酸需要表达的OtopLA在不同的味觉神经元在苍蝇相当于脊椎动物的舌头。在果蝇Drosophila melanogaster中已经发现了苦味、糖和其他味道的受体，而对酸味的广泛调节受体一直是难以捉摸的。先前的工作表明，这样的受体不太可能由果蝇中两个主要味觉受体家族（“味觉受体”和“离子型受体”）之一的基因编码。在这里，为了识别酸味受体，我们测试了编码与哺乳动物Otoprin 1（OTOP 1）质子通道（在小鼠中作为酸味受体）有一定关系的蛋白质的基因的贡献。通过CRISPR/Cas9敲低或突变其中一个基因Otopetrin-Like A（OtopLA），但不影响其他基因（OtopLB或OtopLC），严重损害了对含有高水平HCl或羧酸的甜味溶液的行为排斥，并大大降低了从味觉毛发测量的酸诱导的动作电位。我们从长鼻中分离的OtopLA亚型足以恢复OtopLA突变果蝇的行为敏感性和酸诱导的动作电位放电。在较低浓度下，HCl对果蝇有吸引力，这种吸引力在OtopLA突变体中被消除。细胞类型特异性救援实验表明，OtopLA功能的味觉受体神经元的排斥和吸引高，低水平的质子，分别在不同的子集。这项工作突出了果蝇和哺乳动物中感觉受体的功能保守性，并表明相同的受体可以在食欲和排斥行为中发挥作用。
The taste of acids is critical for animal survival since it enables them to differentiate potentially dangerous from nutritious foods. Due to the general requirement for acid taste for survival, we tested the idea that the receptor mechanism functioning in acid taste may be evolutionarily conserved. Here, we demonstrate that mutation of a Drosophila gene, Otopetrin-Like A (OtopLA), encoding a protein distantly related to the recently identified mammalian acid taste receptor, OTOP1, is essential for both the strong repulsion to highly acidic food and mild attraction to low acidity. The mild attraction and strong aversion to acids requires expression of OtopLA in distinct taste neurons in the fly equivalent of the vertebrate tongue. Receptors for bitter, sugar, and other tastes have been identified in the fruit fly Drosophila melanogaster, while a broadly tuned receptor for the taste of acid has been elusive. Previous work showed that such a receptor was unlikely to be encoded by a gene within one of the two major families of taste receptors in Drosophila, the “gustatory receptors” and “ionotropic receptors.” Here, to identify the acid taste receptor, we tested the contributions of genes encoding proteins distantly related to the mammalian Otopertrin1 (OTOP1) proton channel that functions as a sour receptor in mice. RNA interference (RNAi) knockdown or mutation by CRISPR/Cas9 of one of the genes, Otopetrin-Like A (OtopLA), but not of the others (OtopLB or OtopLC) severely impaired the behavioral rejection to a sweet solution laced with high levels of HCl or carboxylic acids and greatly reduced acid-induced action potentials measured from taste hairs. An isoform of OtopLA that we isolated from the proboscis was sufficient to restore behavioral sensitivity and acid-induced action potential firing in OtopLA mutant flies. At lower concentrations, HCl was attractive to the flies, and this attraction was abolished in the OtopLA mutant. Cell type–specific rescue experiments showed that OtopLA functions in distinct subsets of gustatory receptor neurons for repulsion and attraction to high and low levels of protons, respectively. This work highlights a functional conservation of a sensory receptor in flies and mammals and shows that the same receptor can function in both appetitive and repulsive behaviors.
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DOI：
10.3791/193
发表时间：
2007-01-01
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Shiraiwa, Takashi;Carlson, John R
通讯作者：
Carlson, John R
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
16.6
作者：
LeDue, Emily E.;Chen, Yu-Chieh;Jung, Aera Y.;Dahanukar, Anupama;Gordon, Michael D.
通讯作者：
Gordon, Michael D.
影响因子：
5.3
作者：
Chen YD;Park SJ;Ja WW;Dahanukar A
通讯作者：
Dahanukar A
影响因子：
8.8
作者：
Chen YD;Dahanukar A
通讯作者：
Dahanukar A