Transcription in mouse embryo cells permissively infected by murine cytomegalovirus.
Transcription in mouse embryo cells permissively infected by murine cytomegalovirus.
复制标题
小鼠巨细胞病毒允许感染的小鼠胚胎细胞中的转录。
DOI：
10.1016/0042-6822(83)90550-0
复制
发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Spector,DH
中科院分区：
文献类型：
--
作者：
Marks,JR;Mercer,JA;Spector,DH
分析了小鼠巨细胞病毒(Smith株)感染小鼠胚胎细胞的转录位点和稳定态细胞质病毒RNA的丰度。用32P标记克隆的亚基因组DNA片段，并与含有从感染早期、早期和晚期细胞中提取的多腺苷RNA的过滤器杂交。转录模式在每个时间点都是不同的。即刻-早期转录主要发生在0.770-0.816个地图单位。即刻早期转录的次要位点聚集在0.671-0.861个图谱单位和基因组末端(0.944-0.002个图谱单位)。除了0.278-0.305个图谱单位的片段外，从整个基因组中检测到了早期的转录本。在0.770-0.816个MAP单位的主要即刻-早期转录位点的表达较少，而大多数其他即刻-早期转录位点的浓度在早期时间点升高。早期转录最丰富的位置是0.840-0.861个图单位。感染后期的RNA与所有亚基因组片段杂交。检测到最高浓度的晚期核糖核酸转录本，片段位于0.444-0.770个MAP单位。相反，来自基因组两端的晚期RNA转录本的浓度等于或低于早期时间点的浓度，主要早期位点的晚期RNA浓度(0.840-0.861个MAP单位)显著降低。我们还检测到未感染的小鼠细胞RNA带有三个独立的亚基因组EcoRI片段。
The sites of transcription and abundance of steady-state cytoplasmic viral RNA in murine cytomegalovirus (Smith strain) infected mouse embryp cells were analyzed. Cloned subgenomic DNA fragments were labeled with32P and hybridized to filters containing polyadenylated RNA extracted from cells at immediate-early, early, and late times in the infection. The pattern of transcription was distinctive at each time point. Immediate-early transcription occurred primarily at 0.770–0.816 map units. Minor sites of immediate-early transcription were clustered at 0.671–0.861 map units and at the termini of the genome (0.944-0.002 map units). Early transcripts were detected from the entire genome with the exception of a fragment at 0.278–0.305 map units. The site of major immediate-early transcription at 0.770–0.816 map units was represented less abundantly while the concentration of RNA from most other sites of immediate-early transcription was increased at the early time point. The most abundant site of early transcription was at 0.840–0.861 map units. RNA from late in the infection hybridized to all subgenomic fragments. The highest concentration of late RNA transcripts was detected with fragments located at 0.444–0.770 map units. In contrast, late RNA transcripts from both ends of the genome were present at a concentration equal to or lower than that seen at the early time point, and the concentration of late RNA from the major early site (0.840–0.861 map units) was significantly decreased. We also detected uninfected mouse cell RNA with three separate subgenomicEcoRI fragments.
登录
查看更多内容
人类巨细胞病毒基因表达的转录后控制。
影响因子：
3.7
作者：
DeMarchi,JM
通讯作者：
DeMarchi,JM
影响因子：
3.7
作者：
J. Demarchi
通讯作者：
J. Demarchi
通过显微注射单克隆抗体抑制 SV40 诱导的细胞 DNA 合成。
影响因子：
3.7
作者：
Mercer,WE;Nelson,D;Hyland,JK;Croce,CM;Baserga,R
通讯作者：
Baserga,R
潜伏感染和难以捉摸的巨细胞病毒。
DOI：
--
发表时间：
1983
期刊：
Reviews of Infectious Diseases
影响因子：
--
作者：
M. Jordan
通讯作者：
M. Jordan
鼠巨细胞病毒感染细胞中病毒基因组的计数。
影响因子：
3.7
作者：
V. Misra;M. Muller;J. Hudson
通讯作者：
J. Hudson