The initial deficiency of protein processing and flavonoids biosynthesis were the main mechanisms for the male sterility induced by SX-1 in Brassica napus.
The initial deficiency of protein processing and flavonoids biosynthesis were the main mechanisms for the male sterility induced by SX-1 in Brassica napus.
复制标题
蛋白质加工和类黄酮生物合成的初始缺陷是SX-1诱导甘蓝型油菜雄性不育的主要机制
DOI：
10.1186/s12864-018-5203-y
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发表时间：
2018-11-07
期刊：
影响因子：
4.4
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Ning L;Lin Z;Gu J;Gan L;Li Y;Wang H;Miao L;Zhang L;Wang B;Li M
背景：油菜（Brassica napus）是油菜科油菜属的重要油料作物。化学诱导雄性不育（CIMS）是B中广泛应用的杂种培育方法之一。油菜。识别CIMS中的关键基因和途径对于理解CIMS的分子机制具有重要意义。结果：SX-1处理的B的绒毡层体和弹性体提前退化，绒毡层细胞内堆积异常，花粉壁的tryphine沉积不完全。通过扫描电镜和透射电镜分析，结果表明，SX-1处理的材料早期内质网蛋白质加工和黄酮类化合物生物合成不足。SX-1处理后，花药中激素信号转导、氨基酸、脂肪酸和甾体类化合物的生物合成均受到抑制。同时发现144个转录因子在花药发育早期表达下调，表明B处理的花药和花粉壁发育的早期调控紊乱。油菜。结论：基于多组学整合分析，构建了候选基因和代谢途径的互作网络，为研究SX-1的CHA诱导B雄性不育提供了新的思路。油菜。
Background:Rapeseed (Brassica napus) is an important oil seed crop in the Brassicaceae family. Chemical induced male sterility (CIMS) is one of the widely used method to produce the hybrids in B. napus. Identification of the key genes and pathways that involved in CIMS were important to understand the underlying molecular mechanism. In the present report, a multi-omics integrative analysis, including of the proteomic, transcriptomic and miRNAs, combined with morphological and physiological analysis were conducted.Results:Earlier degeneration of the tapetosomes and elaioplasts, aberrantly stacking in tapetal cells and incompletely deposition in tryphine of pollen wall were observed in chemical hybridization agent (CHA) of SX-1 treated B. napus through SEM and TEM analysis. It was revealed that the deficiencies in protein processing in endoplasmic reticulum (ER) and flavonoids biosynthesis were occurred at early stage in the SX-1 treated materials. Subsequently, plant hormone signal transduction, biosynthesis of amino acids, fatty acids and steroid in anther at later stages were identified down-regulated after SX-1 treatment. 144 transcript factors (TFs) were also indentified to down-regulated at early stage, which suggested the early regulation in anther and pollen wall development were disordered in CHA treated B. napus. In addition, 7 important miRNAs were identified and 2 of the predicted target genes of miRNAs were Rf-like genes.Conclusions:Taken together, an interaction network of candidate genes and the putative metabolism pathways were constructed based on the multi-omics integrative analysis, it provided a new insight into the male sterility induced by CHA of SX-1 in B. napus.
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