Genome-wide analysis of DNA methylation in photoperiod- and thermo-sensitive male sterile rice Peiai 64S.
Genome-wide analysis of DNA methylation in photoperiod- and thermo-sensitive male sterile rice Peiai 64S.
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光热敏感型雄性不育稻培爱64S DNA甲基化全基因组分析
DOI：
10.1186/s12864-015-1317-7
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发表时间：
2015-02-19
期刊：
影响因子：
4.4
通讯作者：
Ding Y
中科院分区：
文献类型：
--
作者：
Hu J;Chen X;Zhang H;Ding Y
表观遗传修饰在植物发育调控中起着重要作用。DNA甲基化是一种重要的表观遗传修饰，在发育过程中动态调节基因的表达。然而，关于水稻光温敏核不育(PTGMS)育性转换过程中甲基化的研究报道很少。本研究利用甲基化DNA免疫沉淀测序技术(MeDIP-seq)比较了水稻光温敏核不育系PA64S在两种不同环境(不同温度和不同日照时长)下的整体DNA甲基化模式。对不育和可育两种不同表型的DNA甲基化分析表明，在PA64S(不育)和PA64S(可育)之间观察到了高甲基化现象，并发现了1258个差异甲基化区域。其中20个差异甲基化基因通过亚硫酸氢盐测序进一步验证，其中4个基因进行了qRT-PCR分析。特别是，编码转录因子BIM2的差异甲基化基因(LOC_Os08g38210)是水稻油菜素类固醇信号转导的一个组成部分。BIM2基因的高甲基化可能抑制油菜素类固醇信号通路的一些下游基因，从而影响PA64S的雄性育性。结果表明，在PA64S(不育)中观察到了高甲基化。基因本体(GO)分析和KEGG分析表明，黄酮醇的生物合成、昼夜节律、光合作用和氧化磷酸化途径参与了PA64S的不育-育性转换。
Epigenetic modifications play important roles in the regulation of plant development. DNA methylation is an important epigenetic modification that dynamically regulates gene expression during developmental processes. However, little studies have been reported about the methylation profiles of photoperiod- and thermo-sensitive genic male sterile (PTGMS) rice during the fertility transition. In this study, using methylated DNA immunoprecipitation sequencing (MeDIP-seq), the global DNA methylation patterns were compared in the rice PTGMS line PA64S under two different environments (different temperatures and day lengths). The profiling of the DNA methylation under two different phenotypes (sterility and fertility) revealed that hypermethylation was observed in PA64S (sterility), and 1258 differentially methylated regions (DMRs) were found between PA64S (sterility) and PA64S (fertility). Twenty differentially methylated genes of them were further validated through bisulfite sequencing, and four of these genes were analyzed by qRT-PCR. Especially, a differentially methylated gene (LOC_Os08g38210), which encoded transcription factor BIM2, is a component of brassinosteroid signaling in rice. The hypermethylated BIM2 gene may suppress some downstream genes in brassinosteroid signaling pathway, and thus affect the male fertility in PA64S. The results presented here indicated that hypermethylation was observed in PA64S (sterility). Gene Ontology (GO) analysis and KEGG analysis revealed that flavone and flavonol biosynthrsis, circadian rhythm, photosynthesis and oxidative phosphorylation pathways were involved in sterility-fertility transition of PA64S.
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影响因子：
9.8
作者：
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