Two molecular weight species of thrombospondin-2 are present in bone and differentially modulated in fractured and nonfractured tibiae in a murine model of bone healing.
Two molecular weight species of thrombospondin-2 are present in bone and differentially modulated in fractured and nonfractured tibiae in a murine model of bone healing.
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DOI：
10.1007/s00223-012-9580-y
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发表时间：
2012-05
影响因子：
4.2
通讯作者：
Sharma, Gorav
中科院分区：
文献类型：
--
作者：
Alford, Andrea I.;Reddy, Anita B.;Goldstein, Steven A.;Murthy, Prithvi;Tayim, Riyad;Sharma, Gorav
我们报告两种免疫反应性的血小板反应蛋白-2（TSP 2），大小约为200和125 kDa，在生长中的长骨，但不完全成熟，小鼠。在体外成骨细胞分泌200 kDa的物质进入培养基早在第3天，它出现在细胞基质层的第7天。一个125-kDa的物种出现在细胞基质层与矿化平行，它是没有检测到在细胞条件培养基。单侧胫骨骨折诱导的创伤部位的200-kDa种类的时间依赖性上调。相比之下，骨折部位125 kDa物质的相对水平低于幼稚对照动物的骨骼。在对侧未接触的对照胫骨中，与从幼稚对照小鼠收获的骨相比，200-kDa物质迅速且大幅减少。水平的125 kDa的物种在未触及的胫骨骨折后随着时间的推移逐渐下降。未受伤的对照骨中的TSP 2基因表达在骨折后21天适度下降。在骨折当天，从未受伤的骨骼中收获的MSC的成骨细胞分化潜能与从幼稚对照动物中收获的那些相比降低。两种异构体的存在表明，TSP 2可能在骨骼组织中进行转录后或翻译后加工。我们的数据还表明，在创伤的背景下，两个TSP 2亚型在骨折愈合的动物受伤和非受伤的骨骼部位差异调制。
We report two immuoreactive species of thrombospondin-2 (TSP2), sized approximately 200 and 125 kDa, in the long bones of growing, but not skeletally mature, mice. In vitro osteoblasts secrete a 200-kDa species into the culture medium as early as day 3, and it appears in the cell-matrix layer by day 7. A 125-kDa species appears in the cell-matrix layer in parallel with mineralization; it is not detected in cell-conditioned medium. Unilateral tibial fracture induced a time-dependent upregulation of the 200-kDa species at the site of trauma. By contrast, relative levels of the 125-kDa species at the fracture site were lower than in bones from naive control animals. In the contralateral untouched control tibia, the 200-kDa species was rapidly and substantially reduced compared to bone harvested from naive control mice. Levels of the 125-kDa species in the untouched tibia declined gradually with time postfracture. TSP2 gene expression in uninjured control bone decreased modestly by 21 days postfracture. On the day of fracture, the osteoblast differentiation potential of MSCs harvested from uninjured bones decreased compared to those harvested from naive control animals. The presence of two isoforms suggests that TSP2 may undergo posttranscriptional or posttranslational processing in skeletal tissue. Our data also suggest that, in the context of trauma, the two TSP2 isforms are differentially modulated at injured and noninjured skeletal sites in an animal undergoing fracture healing.
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影响因子：
1.5
作者：
Boban, Ivana;Barisic-Dujmovic, Tatjana;Clark, Stephen H.
通讯作者：
Clark, Stephen H.
影响因子：
4
作者：
BAB, IA;EINHORN, TA
通讯作者：
EINHORN, TA
缺乏血小板反应蛋白 2 的小鼠表现出结缔组织异常，这与胶原纤维生成紊乱、血管密度增加和出血素质有关。
影响因子：
7.8
作者：
Kyriakides, T R;Zhu, Y H;Smith, L T;Bain, S D;Yang, Z;Lin, M T;Danielson, K G;Iozzo, R V;LaMarca, M;McKinney, C E;Ginns, E I;Bornstein, P
通讯作者：
Bornstein, P
影响因子：
4.8
作者：
GAZIT, D;KARMISH, M;BAB, I
通讯作者：
BAB, I
影响因子：
2.8
作者：
HILTUNEN, A;VUORIO, E;ARO, HT
通讯作者：
ARO, HT