Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation.
Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation.
复制标题
DOI：
10.1038/s41598-021-98233-z
复制
发表时间：
2021-09-29
影响因子：
4.6
通讯作者：
Matsudaira P
中科院分区：
文献类型：
--
作者：
Bhattacharya D;Zhong J;Tavakoli S;Kabla A;Matsudaira P
在斑马鱼胚胎原肠胚形成过程中，胚盘细胞帽组织成左右对称、头尾、背腹极性的胚胎轴向体平面。我们的实验室一直对早期发育的机制感兴趣，并研究了这些大规模的细胞运动是否可以通过基于构造的方法描述为组织水平的机械应变。第一步是通过数字片光显微镜对从中间外延到早期分割的所有细胞核的位置进行成像，将胚胎的表面组织成多细胞球形域，从这些域的运动构建速度场，并从域的密度变化中提取应变率图。在原肠胚形成期间，轴向和赤道方向上的拉伸/膨胀和压缩应变被检测为沿前后轴的前后膨胀沿着和横跨背腹轴的内侧-外侧压缩，并且对应于会聚和伸展的良好表征的形态学运动。原肠胚形成后的应变表现为在分割开始时的局部内侧扩张和在神经胚形成开始时的前部扩张。除了线性应变，旋转/卷曲的对称图案首先检测到在动物半球中的中-epiboly，然后植物半球的原肠胚形成结束。在用C59（一种抑制头部和尾部延伸的Wnt抑制剂）处理的胚胎中，不存在轴向延伸和植物卷曲。通过分析大规模运动的时间序列，整个胚胎的变形可以归因于外延和背侧会聚延伸的组合。
During gastrulation of the zebrafish embryo, the cap of blastoderm cells organizes into the axial body plan of the embryo with left–right symmetry and head–tail, dorsal–ventral polarities. Our labs have been interested in the mechanics of early development and have investigated whether these large-scale cell movements can be described as tissue-level mechanical strain by a tectonics-based approach. The first step is to image the positions of all nuclei from mid-epiboly to early segmentation by digital sheet light microscopy, organize the surface of the embryo into multi-cell spherical domains, construct velocity fields from the movements of these domains and extract strain rate maps from the change in density of the domains. During gastrulation, tensile/expansive and compressive strains in the axial and equatorial directions are detected as anterior and posterior expansion along the anterior–posterior axis and medial–lateral compression across the dorsal–ventral axis and corresponds to the well characterized morphological movements of convergence and extension. Following gastrulation strain is represented by localized medial expansion at the onset of segmentation and anterior expansion at the onset of neurulation. In addition to linear strain, symmetric patterns of rotation/curl are first detected in the animal hemispheres at mid-epiboly and then the vegetal hemispheres by the end of gastrulation. In embryos treated with C59, a Wnt inhibitor that inhibits head and tail extension, the axial extension and vegetal curl are absent. By analysing the temporal sequence of large-scale movements, deformations across the embryo can be attributed to a combination of epiboly and dorsal convergence-extension.
登录
查看更多内容
影响因子：
25
作者：
Koser DE;Thompson AJ;Foster SK;Dwivedy A;Pillai EK;Sheridan GK;Svoboda H;Viana M;Costa LD;Guck J;Holt CE;Franze K
通讯作者：
Franze K
影响因子：
56.9
作者：
Xu, Peng-Fei;Houssin, Nathalie;Thisse, Christine
通讯作者：
Thisse, Christine
母体葫芦娃通过脊椎动物中的β-连环蛋白决定胚胎体轴
影响因子：
56.9
作者：
Yan, Lu;Chen, Jing;Meng, Anming
通讯作者：
Meng, Anming
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
11.8
作者：
Lekven, AC;Thorpe, CJ;Moon, RT
通讯作者：
Moon, RT